Recombinant Protein

PDCD1 / CD279 / PD-1

Human

Preservative Free. Also available Unmodified.

52 kDa

CHO Cells

Hamster-Chinese

A soluble fusion protein consisting of the extracellular (139aa) domain of human CD279(PD-1) and murine IgG2a Fc +hinge. In SDS-PAGE, this protein runs at 52 kd under native, and 27 kd under reducing conditions. Data suggest that the murine Ig Fc portion of this construct may be prone to cleavage. Five x 10^5cultured human monocyte-derived Dendritic cells (moDC) were washed and incubated 45 minutes on ice with 80 ul of CD279-muIg/Biotin at 10 µg/ml. Cells were washed twice and incubated with secondary reagent Streptavidin/PE, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.57 log10 fluorescent units. This binding was blocked when cells were pre incubated with 100 ug/ml of purified CD279-muIg.

Not Tested

Not Tested

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl

Store at 2°C to 5°C for up to 6 months. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

Uniprot: Q15116 NCBI: NM_005018 NP_005009.2