NT5DC1 Protein LS-G86877 is Over-Expression Lysate Human NT5DC1 in Human with Myc + DDK tag(s).
Over-Expression Lysate Protein
Predicted Molecular Weight
51.7 kDa Full Length
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human NT5DC1 (NM_152729) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Reconstitute with SDS Sample Buffer
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
Western blot: Spin down briefly and resuspend in 2X SDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT). Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
For research use only.