Target
IL22
Synonyms
IL22 | Cytokine Zcyto18 | ILTIF | Interleukin 22 | IL-22 | IL-D110 | TIFIL-23 | Zcyto18 | IL-TIF | Interleukin-22
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 147 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 16,880 Da. The DTT-reduced protein migrates at approximately 15kDa and the non-reduced protein migrates at approximately 14kDa by SDS-PAGE.
Purification
Greater than 98% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
The ED50 is 0.062-0.177 ng/ml, corresponding to a specific activity of 1.61-0.56 X10^7 units/mg, as determined by a dose dependent stimulation of human Colo205 cells in production of IL-10.
Endotoxin
Less than 0.01 ng/µg (Less than 0.1 EU/µg) (determined by LAL method).
Presentation
0.22 µm filtered protein solution, pH 7.2, 10 mM NaH2PO4, 150 mM NaCl
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee