IFN Gamma / Interferon Gamma Protein LS-G3635 is Recombinant Human IFN Gamma / Interferon Gamma in E. coli It is biologically active and is low in endotoxin Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg)..
IFN Gamma / Interferon Gamma
IFN-gamma is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-gamma receptors are present in most immune cells, which respond to IFN-gamma signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-gamma signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-gamma stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-gamma species-specific and is biologically active only in human and primate cells. Recombinant human IFN-gamma is a 16.8 kDa protein containing 144 amino acid residues.
Greater than 98% by SDS-PAGE and HPLC
Assay 1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED50 for this effect is 5.0-10.0 ng/ml. Assay 2: The ED50 as determined by a cytotoxicity Assay using HT-29 cells is = 0.05 ng/ml, corresponding to a specific activity of = 2 x 10^7 units/mg.
Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg).
Lyophilized from 10mM sodium phosphate, pH 7.4.
Reconstitute in distilled water to a concentration of 1.0 mg/mL
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
For research use only.
About IFN Gamma / Interferon Gamma