FGF2 / Basic FGF Protein LS-G23535 is Recombinant Human FGF2 / Basic FGF in E. coli It is biologically active and is low in endotoxin Less than 1.0 EU/µg protein (determined by LAL method)..
Predicted Molecular Weight
Basic fibroblast growth factor (bFGF), also known as FGF2, is expressed by endothelial cells and is a mediator of angiogenesis. bFGF also has cardioprotective functions during heart injury. The application of bFGF is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state. Recombinant bFGF-154aa is the full length bFGF protein encoded by the human FGF2 gene. bFGF-154aa is the most popular tissue culture product at Shenandoah Biotechnology Inc. There are no detectable differences in biological activity between bFGF-154aa and the truncated bFGF-147aa proteins. This product is produced with no animal or human origin raw products. All processing and handling employs animal free equipment and animal free protocols.
Greater than 95% by SDS-PAGE
Tested using: 3T3 Proliferation. Less than or equal to 5 ng/mL and greater than or equal to 2.0 x 10^5 units/mg. Typical ED50 is less than 1 ng/mL.
Less than 1.0 EU/µg protein (determined by LAL method).
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5
Reconstitute in sterile water to 0.1 mg/ml
Store at -20°C to -80°C for up to 1 year. Store at 4°C for up to 1 month or up to 3 months at -20°C to -80°C after reconstitution.
Upon reconstitution, a small amount of visible precipitate can be expected. A 10% overfill has been added to the total material vialed to compensate for this loss.
For research use only.