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Catalog Number Size Price
LS-G137667-10 10 µg (0.2 mg/ml) $268 
LS-G137667-25 25 µg (0.2 mg/ml) $322 
LS-G137667-100 100 µg $436 
FGF1 / Acidic FGF Protein - NIH3T3 proliferation induced by human FGF-1 in the presence of 10 µg/ml heparin.

Human FGF1 / Acidic FGF Protein (Recombinant) - LS-G137667

Human FGF1 / Acidic FGF Protein (Recombinant) - LS-G137667

Description:
FGF1 / Acidic FGF Protein LS-G137667 is a Recombinant Human FGF1 / Acidic FGF. It is biologically active and is low in endotoxin; Less than 0.01 ng/µg (determined by LAL method). For Research Use Only
Price
Catalog Number
$268
LS-G137667-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
FGF1 / Acidic FGF Protein LS-G137667 is a Recombinant Human FGF1 / Acidic FGF. It is biologically active and is low in endotoxin; Less than 0.01 ng/µg (determined by LAL method). For Research Use Only

Specifications

Type
Recombinant Protein
Target
FGF1 / Acidic FGF
Synonyms
FGF1 | AFGF | Endothelial cell growth factor | Fibroblast growth factor 1 | FGFA | ECGFB | HBGF-1 | GLIO703 | ECGF | ECGF-beta | ECGFA | FGF-1 | FGF-alpha | HBGF1
Species
Human
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 140 amino acid recombinant protein has a predicted molecular mass of approximately 16 kD. The DTT-reduced and non-reduced protein migrates at approximately 17 kD by SDS-PAGE. The predicted N-terminal amino acid is Met.
Source Species
E. coli
Purification
Greater than 97% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
The ED50 is 0.2 - 1.0 ng/ml, corresponding to a specific activity 1.0 - 5.0 x 10^6 units/mg, as determined by a dose-dependent stimulation of NIH3T3 cell proliferation in the presence of 10 µg/ml heparin.
Endotoxin
Less than 0.01 ng/µg (determined by LAL method).
Presentation
0.22 µm filtered protein solution in 20 mM MOPS, pH 7.0, 100 mM NaCl
Storage
Store unopened at -20°C for 3 months, or at -70°C for up to 6 months. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About FGF1 / Acidic FGF
P05230 X59065 P05230

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Images

Proliferation

FGF1 / Acidic FGF Protein - NIH3T3 proliferation induced by human FGF-1 in the presence of 10 µg/ml heparin.
NIH3T3 proliferation induced by human FGF-1 in the presence of 10 µg/ml heparin.

Proliferation

FGF1 / Acidic FGF Protein - NIH3T3 proliferation induced by human FGF-1 in the presence of 10 µg/ml heparin.
NIH3T3 proliferation induced by human FGF-1 in the presence of 10 µg/ml heparin.

Proliferation

FGF1 / Acidic FGF Protein - NIH3T3 proliferation induced by human FGF-1 in the presence of 10 µg/ml heparin.
NIH3T3 proliferation induced by human FGF-1 in the presence of 10 µg/ml heparin.

Proliferation

FGF1 / Acidic FGF Protein - NIH3T3 proliferation induced by human FGF-1 in the presence of 10 µg/ml heparin.
NIH3T3 proliferation induced by human FGF-1 in the presence of 10 µg/ml heparin.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024