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Catalog Number Size Price
LS-G3808-10 10 µg $379 
LS-G3808-50 50 µg $750 
DLL1 Protein - Adipogenesis inhibition of 3T3L1 cells.3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM dexamethasone, 0.5mM IBMX, 10 ug/ml insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml hDLL1-Fc and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
DLL1 Protein - Adipogenesis inhibition. Macroscopic pictures.
DLL1 Protein - Induction of Hes-1 with the treatment of recombinant human DLL1-Fc (Prod. No. AG-40A-0116Y). A mouse preadipocyte cell line, 3T3L1, was stimulated with 5ug/ml of human DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
DLL1 Protein - Interaction of human Notch1 with human DLL1. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL1-Fc FITC conjugate for DLL1-Fc binding.
DLL1 Protein - Adipogenesis inhibition of 3T3L1 cells.3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM dexamethasone, 0.5mM IBMX, 10 ug/ml insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml hDLL1-Fc and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
DLL1 Protein - Adipogenesis inhibition. Macroscopic pictures.
DLL1 Protein - Induction of Hes-1 with the treatment of recombinant human DLL1-Fc (Prod. No. AG-40A-0116Y). A mouse preadipocyte cell line, 3T3L1, was stimulated with 5ug/ml of human DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
DLL1 Protein - Interaction of human Notch1 with human DLL1. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL1-Fc FITC conjugate for DLL1-Fc binding.
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Human DLL1 Protein (Recombinant Human IgG1 Fc) (aa1-545) - LS-G3808

Human DLL1 Protein (Recombinant Human IgG1 Fc) (aa1-545) - LS-G3808

Description:
DLL1 Protein LS-G3808 is a Recombinant Human DLL1 produced in CHO Cells aa 1-545 with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.01 EU/µg protein (determined by LAL method).
Price
Catalog Number
$379
LS-G3808-10
Toll Free North America
866-819-4732
For Research Use Only

Overview

Description:
DLL1 Protein LS-G3808 is a Recombinant Human DLL1 produced in CHO Cells aa 1-545 with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.01 EU/µg protein (determined by LAL method).

Specifications

Type
Recombinant Protein
Target
DLL1
Synonyms
DLL1 | Delta-like 1 (Drosophila) | Delta (Drosophila)-like 1 | Drosophila Delta homolog 1 | Delta-like protein 1 | Delta1 | H-Delta-1 | DL1 | Npa
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Tag
Human IgG1 Fc
Region
aa 1-545
Predicted Molecular Weight
~100kDa (SDS-PAGE)
Expression System
CHO Cells
Source Species
Hamster-Chinese
Purification
Greater than 90% by SDS-PAGE
Usage Summary
Reconstitute the 70 ug size in 100 ul sterile water. Reconstitute the 50 ug size in 50 ul of sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.
Bio-Activity
Inhibits adipogenesis of 3T3L-1 cells. Induces Hes-1 in 3T3L-1 cells.
Endotoxin
Less than 0.01 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from PBS
Reconstitution
Reconstitute with 50 µl sterile water.
Storage
Store lyophilized at -20°C for up to 1 year. Once reconstituted store at 4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About DLL1
O00548 NM_005618 NP_005609.3

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Images

Functional Assay

DLL1 Protein - Adipogenesis inhibition of 3T3L1 cells.3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM dexamethasone, 0.5mM IBMX, 10 ug/ml insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml hDLL1-Fc and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Adipogenesis inhibition of 3T3L1 cells.3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM dexamethasone, 0.5mM IBMX, 10 ug/ml insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml hDLL1-Fc and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.

Functional Assay

DLL1 Protein - Adipogenesis inhibition. Macroscopic pictures.
Adipogenesis inhibition. Macroscopic pictures.

Functional Assay

DLL1 Protein - Induction of Hes-1 with the treatment of recombinant human DLL1-Fc (Prod. No. AG-40A-0116Y). A mouse preadipocyte cell line, 3T3L1, was stimulated with 5ug/ml of human DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
Induction of Hes-1 with the treatment of recombinant human DLL1-Fc (Prod. No. AG-40A-0116Y). A mouse preadipocyte cell line, 3T3L1, was stimulated with 5ug/ml of human DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.

Functional Assay

DLL1 Protein - Interaction of human Notch1 with human DLL1. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL1-Fc FITC conjugate for DLL1-Fc binding.
Interaction of human Notch1 with human DLL1. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL1-Fc FITC conjugate for DLL1-Fc binding.

Functional Assay

DLL1 Protein - Adipogenesis inhibition of 3T3L1 cells.3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM dexamethasone, 0.5mM IBMX, 10 ug/ml insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml hDLL1-Fc and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Adipogenesis inhibition of 3T3L1 cells.3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM dexamethasone, 0.5mM IBMX, 10 ug/ml insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml hDLL1-Fc and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.

Functional Assay

DLL1 Protein - Adipogenesis inhibition. Macroscopic pictures.
Adipogenesis inhibition. Macroscopic pictures.

Functional Assay

DLL1 Protein - Induction of Hes-1 with the treatment of recombinant human DLL1-Fc (Prod. No. AG-40A-0116Y). A mouse preadipocyte cell line, 3T3L1, was stimulated with 5ug/ml of human DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
Induction of Hes-1 with the treatment of recombinant human DLL1-Fc (Prod. No. AG-40A-0116Y). A mouse preadipocyte cell line, 3T3L1, was stimulated with 5ug/ml of human DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.

Functional Assay

DLL1 Protein - Interaction of human Notch1 with human DLL1. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL1-Fc FITC conjugate for DLL1-Fc binding.
Interaction of human Notch1 with human DLL1. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL1-Fc FITC conjugate for DLL1-Fc binding.

Functional Assay

DLL1 Protein - Adipogenesis inhibition of 3T3L1 cells.3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM dexamethasone, 0.5mM IBMX, 10 ug/ml insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml hDLL1-Fc and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Adipogenesis inhibition of 3T3L1 cells.3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM dexamethasone, 0.5mM IBMX, 10 ug/ml insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml hDLL1-Fc and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.

Functional Assay

DLL1 Protein - Adipogenesis inhibition. Macroscopic pictures.
Adipogenesis inhibition. Macroscopic pictures.

Functional Assay

DLL1 Protein - Induction of Hes-1 with the treatment of recombinant human DLL1-Fc (Prod. No. AG-40A-0116Y). A mouse preadipocyte cell line, 3T3L1, was stimulated with 5ug/ml of human DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
Induction of Hes-1 with the treatment of recombinant human DLL1-Fc (Prod. No. AG-40A-0116Y). A mouse preadipocyte cell line, 3T3L1, was stimulated with 5ug/ml of human DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.

Functional Assay

DLL1 Protein - Interaction of human Notch1 with human DLL1. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL1-Fc FITC conjugate for DLL1-Fc binding.
Interaction of human Notch1 with human DLL1. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL1-Fc FITC conjugate for DLL1-Fc binding.

Functional Assay

DLL1 Protein - Adipogenesis inhibition of 3T3L1 cells.3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM dexamethasone, 0.5mM IBMX, 10 ug/ml insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml hDLL1-Fc and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Adipogenesis inhibition of 3T3L1 cells.3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM dexamethasone, 0.5mM IBMX, 10 ug/ml insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml hDLL1-Fc and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.

Functional Assay

DLL1 Protein - Adipogenesis inhibition. Macroscopic pictures.
Adipogenesis inhibition. Macroscopic pictures.

Functional Assay

DLL1 Protein - Induction of Hes-1 with the treatment of recombinant human DLL1-Fc (Prod. No. AG-40A-0116Y). A mouse preadipocyte cell line, 3T3L1, was stimulated with 5ug/ml of human DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
Induction of Hes-1 with the treatment of recombinant human DLL1-Fc (Prod. No. AG-40A-0116Y). A mouse preadipocyte cell line, 3T3L1, was stimulated with 5ug/ml of human DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.

Functional Assay

DLL1 Protein - Interaction of human Notch1 with human DLL1. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL1-Fc FITC conjugate for DLL1-Fc binding.
Interaction of human Notch1 with human DLL1. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL1-Fc FITC conjugate for DLL1-Fc binding.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/19/2021