Complement C5a Protein LS-G136255 is Recombinant Human Complement C5a in E. coli It is biologically active and is low in endotoxin Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg)..
Predicted Molecular Weight
Complement 5a (C5a) is an enzymatically generated glycoprotein belonging to the anaphylatoxin family of structurally and functionally related proteins. Generated upon the activation of the complement system, C5a, together with C4a, C3a, and the membrane attack complex (C5b-9), functions as a central player in host defense by inducing smooth muscle cell contraction, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes through cell degranulation. In addition to acting as a direct mediator of localized inflammatory response, C5a also initiates both the synthesis and release of IL-8 from monocytes and bronchial epithelial cells, stimulates the proliferation of neurons and hepatocytes, and functions as a potent chemoattractant. Where C5a deficiency, a rare defect of the complement pathway caused by the mutation of the C5a gene, is associated with susceptibility to severe infections, excessive C5a activation has been linked to liver fibrosis, sepsis, adult respiratory distress syndrome, rheumatoid arthritis, Alzheimer’s disease, and ischemic heart disease. Human C5a shares 60% and 54% sequence identity to mouse and rat C5a, respectively. The human C5 gene encodes a 1,676 amino acid glycoprotein that is comprised of a disulfide-linked C5 alpha and a C5 beta chain, the former of which contains the active, 74 amino acid C5a anaphylatoxin chain. Recombinant Human C5a is an 8.3 kDa glycoprotein containing the 74 amino acid residues of the C5a anaphylatoxin chain.
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Measured by its ability to support adhesion of Human Umbilical Vein Endothelial Cells (HUVEC) to the Recombinant Human C5a-coated surface.
Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg).
Lyophilized from sterile filtered 10mM Tris, pH 7.5
Centrifuge vial prior to opening. Do not vortex. Reconstitute with H2O to 0.1-1.0 mg/ml.
Before reconstitution: Short term: Room temperature; Long term: -20°C to -80°C. After reconstitution: Short term: 4°C; Long term: Aliquot and store with a carrier protein at -20°C to -80°C. Avoid freeze thaw cycles. Microcentrifuge before opening.
For research use only.