Target
CD28
Synonyms
CD28 | CD28 antigen | CD28 antigen (Tp44) | Tp44 | CD28 molecule
Modifications
Unmodified.
Also available
Preservative Free.
Conjugations
Unconjugated.
Also available conjugated with
Biotin.
Region
Extracellular domain of human CD28 fused to murine IgG2a Fc.
Expression System
CHO Cells
Source Species
Hamster-Chinese
Usage Summary
A soluble fusion protein consisting of the extracellular (134aa) domain of human CD28 fused to murine IgG2a Fc (233aa). CD28-muIg was tested for activity and quantitated in EIA. Fusion protein was captured using Goat-anti-mouse antibody coated plate. After 45 minute incubation, plates were washed twice and an excess of Mouse Ig was added to completely block the plate. Anti-CD28/Biotin (1 µg/ml) followed by Streptavidin/HRP, and TMB/H2O2 were used for detection. Fusion protein was detectable at 4 ng/ml in this assay.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin Sulfate
Storage
Store at 2°C to 5°C for up to 1 year. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee