Target
CD28
Synonyms
CD28 | CD28 antigen | CD28 antigen (Tp44) | Tp44 | CD28 molecule
Conjugations
Unconjugated
Tag
Human IgG1 Fc, C-terminus
Predicted Molecular Weight
66-70 kDa
AA Sequence
NKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKPexpressedwithaC-terminalhumanIgG1Fcfragment
Expression System
HEK 293 Cells
Purification
Greater than 97% by SDS-PAGE
Bio-Activity
Immobilized human CD28 Fcat 2µg/mL (100µL/well) can bind human Biotin-B7-1(CD80) Fc with a linear range of 0.01-0.5µg/mL when detected by Streptavidin-HRP second antibody.
Endotoxin
Less than 0.2 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from a 0.2 um filtered solution in PBS
Reconstitution
Reconstitute in ddH2O or PBS at 100 ug/ml.
Storage
Store lyophilized at -70°C or below for up to 6 months. Store reconstituted at 4°C for up to 1 week or at -20°C for up to 3 months. Add a carrier protein (0.1% BSA) for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee