Target
CD274 / B7-H1 / PD-L1
Synonyms
CD274 | B7-H | B7 homolog 1 | B7-H1 | B7H1 | CD274 molecule | CD274 antigen | PDCD1 ligand 1 | PDL1 | PD-L1 | PDCD1L1 | PDCD1LG1 | Programmed death ligand 1
Modifications
Preservative Free.
Also available
Unmodified.
Conjugations
Unconjugated
Region
Murine CD8 alpha leader sequence, the mature extracellular domain of human CD274 fused to murine IgG
Predicted Molecular Weight
54.4 kDa
AA Sequence
ftvtvpkdlyvveygsnmtieckfpvekqldlaalivywemedkniiqfvhgeedlkvqhssyrqrarllkdqlslgnaalqitdvklqdagvyrcmisyggadykritvkvnapynkinqrilvvdpvtseheltcqaegypkaeviwtssdhqvlsgkttttnskreeklfnvtstlrintttneifyctfrrldpeenhtaelvipelplahppnerthtr
Expression System
CHO Cells
Source Species
Hamster-Chinese
Usage Summary
A soluble fusion protein consisting of the murine CD8 alpha leader sequence, the mature extracellular (224aa) domain of human CD274 fused to murine IgG2a Fc + hinge (233aa). muCD8 apha signal peptide residual amino acids+ linker: (1)kpqapelrgsas, CD274 mature EC (224aa): (13)ftvtvpkdlyvveygsnmtieckfpvekqldlaalivywemedkniiqfvhgeedlkvqhssyrqrarllkdqlslgnaalqitdvklqdagvyrcmisyggadykritvkvnapynkinqrilvvdpvtseheltcqaegypkaeviwtssdhqvlsgkttttnskreeklfnvtstlrintttneifyctfrrldpeenhtaelvipelplahppnerthtr, Linker + Murine IgG2a Hinge + Fc (235 aa): (237)gteprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgkefkckvnnkdlpapiertiskpkgsvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwvernsyscsvvheglhnhhttksfsrtpg(471). Predicted monomeric (non glycosylated) molecular weight: 54.4 kd. The molecule is dimeric and runs at about 120 kd in SDS-PAGE under native conditions. Purity was >90% by SDS-PAGE. N-terminal sequence was as predicted: KPQAP. Recombinant protein was detectable in EIA by Biotinylated antibody ANC6H1 as well as clone M1H1.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl
Storage
Store at 2°C to 5°C for up to 6 months. Avoid freeze-thaw cycles. Open under aseptic conditions.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD274 / B7-H1 / PD-L1