Target
CD244
Synonyms
CD244 | 2B4 | H2B4 | NAIL | NKR2B4 | Nmrk | SLAMF4 | CD244 antigen
Modifications
Unmodified.
Also available
Preservative Free.
Conjugations
Unconjugated.
Also available conjugated with
Biotin.
Region
Murine CD8 alpha signal peptide residual amino acids and linker.
Predicted Molecular Weight
49.5 kDa
AA Sequence
qgsadhvvsisgvplqlqpnsiqtkvdsiawkkllpsqngfhhilkwengslpsntsndrfsfivknlsllikaaqqqdsglyclevtsisgkvqtatfqvfvfdkvekprlqgqgkildrgrcqvalsclvsrdgnvsyawyrgskliqtagnltyldeevdingthtytcnvsnpvsweshtlnltqdcqnahqefr
Expression System
CHO Cells
Source Species
Hamster-Chinese
Usage Summary
A soluble molecule consisting of murine CD8 alpha signal peptide residual amino acids and linker: (1)kpqapegkgc(10), the mature extracellular domain of human CD244 (199aa): (11)qgsadhvvsisgvplqlqpnsiqtkvdsiawkkllpsqngfhhilkwengslpsntsndrfsfivknlsllikaaqqqdsglyclevtsisgkvqtatfqvfvfdkvekprlqgqgkildrgrcqvalsclvsrdgnvsyawyrgskliqtagnltyldeevdingthtytcnvsnpvsweshtlnltqdcqnahqefr(209), Murine IgG2a Fc + hinge regions: (233aa)eprgptikpcppckcpapnllggpsvfifppkikdvlmislspivtcvvvdvseddpdvqiswfvnnvevhtaqtqthredynstlrvvsalpiqhqdwmsgkefkckvnnkdlpapiertiskpkgsvrapqvyvlpppeeemtkkqvtltcmvtdfmpediyvewtnngktelnykntepvldsdgsyfmysklrvekknwvernsyscsvvheglhnhhttksfsrtpgk (442aa total). The molecule has a predicted monomeric non glycosylated molecular weight of 49.5 kd. The molecule is dimeric. In SDS-PAGE, it runs at apporoximately 135 kD native, and 70 kD reduced. CD244-muIg binds to recombinant CD48 in EIA and cell surface CD48 on Raji cell surface in FACS. Recombinant soluble CD244-muIg was tested for binding in FACS. Five x 10^5 cultured human Raji cells per tube were washed and incubated 45 minutes on ice with 80 µl of Recombinant CD244-muIg at a concentration of 1 µg/ml. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.40 log10fluorescent units when compared to muIgFc Control Protein.
Presentation
50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin Sulfate
Storage
Store at 2°C to 5°C for up to 1 year. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee