Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
ACE2 Protein, ACE-related carboxypeptidase Protein, ACEH Protein, Metalloprotease MPROT15 Protein
Angiotensin-Converting Enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII.
Hydrolysis of a fluorescent peptide substrate, Mca-Y-V-A-D-A-P-K(Dnp)-OH. To each test tube, 2ml of assay buffer (75mM Tris, pH 7.4, 1M NaCl, 10 uM zinc chloride, 10 uM fluorescent peptide substrate) was added. Reactions were initiated by the addition of 20 ul (1 ug/ml, final 10ng/ml) of human ACE2-FLAG (Prod. No. A-40A-0048), a FLAG-tag control protein, and buffer only (for blank). Reactions were performed for 20 minutes at room temperature and stopped by the addition of 0.2ml 500mM EDTA. The fluorescence intensity was measured using luminescence spectrometer (excitation = 320nm, emission = 405nm).