HL-60 Cells Protein LS-G133890 is Cell/Tissue Lysate HL-60 Cells in Human
Cell/Tissue Lysate Protein
This product includes 1 vial containing 100µg of HL60 (Human Acute Promyelocytic Leukemia) Whole Cell Lysate in Modified RIPA buffer(25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1x Proteinase inhibitor cocktail mix (Sigma) and 1mM Na3VO4) and 1 vial containing 250µl of a reducing buffer (2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)) for SDS-PAGE gel loading.
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma) and 1mM Na3VO4
Store undiluted at -20°C for up to 1 year or diluted at -80°C for up to 1 year. Avoid repeated freeze-thaw cycles.
Lysate samples can be diluted 1:1 with 2xSDS Sample Buffer provided. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
For research use only.
Available for shipment within the USA only.