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FliC / Flagellin Native Protein LS-G3883


Wt. Vol. Conc. Price
10 µg - - $265
Inquire for larger quantities
LSBio (Direct) LSBio (Direct)

Most Popular FliC / Flagellin Proteins

FliC / Flagellin Native Protein - LS-G3927
Salmonella typhimurium Expression System
Unpurified / Endotoxin Level: Less than 0.1 EU/µg protein (determined by LAL method).
Functional Assay Image
Salmonella typhimurium FliC / Flagellin Recombinant Protein - LS-G18909
E. coli Expression System
E. coli FliC / Flagellin Protein - LS-G51774

100% Guaranteed 100% Guaranteed
Native Protein
FliC / Flagellin
Salmonella typhimurium
Salmonella typhimurium
Greater than 95% by SDS-PAGE
Activation of TLR5 in human epithelial cell assays based on NF-?B luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, ß-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1µg /mouse
Less than 0.01 EU/µg protein (determined by LAL method).
Lyophilized from PBS.
Reconstitute with 100 µl sterile water.
Store lyophilized at -20°C for at least 6 months. Once reconstituted store at +4°C for immediate use.
For research use only.

About FliC / Flagellin

Functional Assay

Functional Assay
Flagellin (AG-40B-0025) activates TLR5 in vivo. Method: Mice WT are injected i.v. with indicated doses of Flagellin (Prod. No. AG-40B-0025). After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin (Prod. No. AG-40B-0025), but no expression of CCL20 and IL-6 could be observed (not shown).

Requested From: United States
Date Requested: 10/28/2016

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