Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Activation of TLR5 in human epithelial cell assays based on NF-?B luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, ß-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1µg /mouse
Less than 0.01 EU/µg protein (determined by LAL method).
Lyophilized from PBS.
Reconstitute with 100 µl sterile water.
Store lyophilized at -20°C for at least 6 months. Once reconstituted store at +4°C for immediate use.
Flagellin (AG-40B-0025) activates TLR5 in vivo. Method: Mice WT are injected i.v. with indicated doses of Flagellin (Prod. No. AG-40B-0025). After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10ug of Flagellin (Prod. No. AG-40B-0025), but no expression of CCL20 and IL-6 could be observed (not shown).