Recombinant Protein

Polymyxin resistance protein MCR-1

E. coli

Unmodified

76.1kDa

E. coli

E. coli

Greater than 90% by SDS-PAGE

Probably catalyzes the addition of a phosphoethanolamine moiety to lipid A. Phosphoethanolamine modification of lipid A gives polymyxin resistance (PubMed:26603172).1 Publication Confers resistance to polymyxin-type antibiotics; expression of the Mcr-1 protein in E.coli increases colistin and polymyxin B minimal inhibitory concentration (MIC) from 0.5 mg/ml to 2.0 mg/ml. The pHNSHP45 plasmid can transfer efficiently (0.1 to 0.001) to other E.coli strains by conjugation and increases polymxin MIC by 8- to 16-fold; it may not require selective pressure to be maintained in the cell. When transformed into K.pneumoniae or P.aeruginosa it also increases polymxin MIC 8- to 16-fold. In a murine (BALB/c mice) thigh infection study using an mcr1-encoding plasmid isolated from a human patient, the plasmid confers in vivo protection against colistin (PubMed:26603172).

Not Tested

Not Tested

Tris, 50% Glycerol

Store lyophilized at -20°C or -80°C for up to 1 year. Once reconstituted: store undiluted at 4°C for up to 1 week. Aliquot and store undiluted at -20°C or -80°C for up to 6 months. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

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