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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-G145868-100 100 µg $345 
SARS-CoV-2 S1 Protein - Recombinant 2019-nCoV Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.
SARS-CoV-2 S1 Protein - Immobilized Recombinant Human ACE2 at 2µg/mL (100 µL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL
SARS-CoV-2 S1 Protein - Recombinant 2019-nCoV Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.
SARS-CoV-2 S1 Protein - Immobilized Recombinant Human ACE2 at 2µg/mL (100 µL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL
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Coronavirus SARS-CoV-2 SARS-CoV-2 S1 Protein (Recombinant Human IgG Fc-6His, C-terminus) (Val11-Arg682) - LS-G145868

Coronavirus SARS-CoV-2 SARS-CoV-2 S1 Protein (Recombinant Human IgG Fc-6His, C-terminus) (Val11-Arg682) - LS-G145868

Description:
SARS-CoV-2 S1 Protein LS-G145868 is a Recombinant Coronavirus SARS-CoV-2 SARS-CoV-2 S1 produced in HEK 293 Cells Val11-Arg682 with Human IgG Fc-6His, C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method).
Price
Catalog Number
$345 
LS-G145868-100
100 µg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Description:
SARS-CoV-2 S1 Protein LS-G145868 is a Recombinant Coronavirus SARS-CoV-2 SARS-CoV-2 S1 produced in HEK 293 Cells Val11-Arg682 with Human IgG Fc-6His, C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method).

Specifications

Type
Recombinant Protein
Target
SARS-CoV-2 S1
Synonyms
2019-nCoV S1 Glycoprotein | Wuhan Coronavirus S1 Glycoprotein | SARS-Cov2 (COVID-19) S1 Glycoprotein | SARS-CoV-2 | SARS-Cov2 | COVID-19
Species
Coronavirus SARS-CoV-2
Modifications
Unmodified
Conjugations
Unconjugated
Tag
Human IgG Fc-6His, C-terminus
Region
Val11-Arg682
Expression System
HEK 293 Cells
Source Species
Human
Purification
Greater than 95% by SDS-PAGE
Usage Summary
Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Arg682) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with an Fc, 6×His tag at the C-terminus.
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized nCoV-S1 at 2 g/mL (100 L/well) can bind Recombinant Human ACE2 with a linear range of 0.06-0.3µg/mL.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from a sterile solution in PBS, pH 7.4.
Storage
Short term: Store at 2-8 °C for up to 1 week or -20 °C for 3 months. Long term: Store lyophilized at -20°C to -80 °C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee

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Images

Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis

SARS-CoV-2 S1 Protein - Recombinant 2019-nCoV Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.
Recombinant 2019-nCoV Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.

Enzyme-Linked Immunosorbent Assay

SARS-CoV-2 S1 Protein - Immobilized Recombinant Human ACE2 at 2µg/mL (100 µL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL
Immobilized Recombinant Human ACE2 at 2µg/mL (100 µL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL

Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis

SARS-CoV-2 S1 Protein - Recombinant 2019-nCoV Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.
Recombinant 2019-nCoV Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.

Enzyme-Linked Immunosorbent Assay

SARS-CoV-2 S1 Protein - Immobilized Recombinant Human ACE2 at 2µg/mL (100 µL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL
Immobilized Recombinant Human ACE2 at 2µg/mL (100 µL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL

Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis

SARS-CoV-2 S1 Protein - Recombinant 2019-nCoV Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.
Recombinant 2019-nCoV Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.

Enzyme-Linked Immunosorbent Assay

SARS-CoV-2 S1 Protein - Immobilized Recombinant Human ACE2 at 2µg/mL (100 µL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL
Immobilized Recombinant Human ACE2 at 2µg/mL (100 µL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL

Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis

SARS-CoV-2 S1 Protein - Recombinant 2019-nCoV Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.
Recombinant 2019-nCoV Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.

Enzyme-Linked Immunosorbent Assay

SARS-CoV-2 S1 Protein - Immobilized Recombinant Human ACE2 at 2µg/mL (100 µL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL
Immobilized Recombinant Human ACE2 at 2µg/mL (100 µL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL

Requested From: United States
Date Requested: 5/25/2020