Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL4-His at 10 µg/mL (100 µl/well) can bind rat IL4R-Fc, The EC50 of rat IL4R-Fc is 0.12-0.29 µg/mL. 2. Measured by its ability to inhibit mIL4-dependent proliferation of HT-2 cells. The ED50 for this effect is typically 2-10 ng/ml in the presence of 2 ng/ml Recombinant Mouse IL4.
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.5.
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine.