Synonyms
ACO1 | ACONS | Aconitase | Aconitase 1, soluble | Aconitate hydratase | Citrate hydro-lyase | IREB1 | IRE-BP 1 | IREBP | IRP1 | Ferritin repressor protein | IREBP1 | Iron regulatory protein 1
Reconstitution
Add distilled water/PBS to give a target peptide concentration of 1-5mg/mL, and sonicate. To any insoluble peptide add pure acetic acid to bring the concentration of acetic acid to 10% (v/v), and sonicate. If the peptide remains insoluble, add acetonitrile to 20% (v/v), and sonicate. If the peptide again remains insoluble, lyophilize the peptide and add neat DMF dropwise until the peptide dissolves. Dilute this solution slowly with water to give approximately 10% (v/v) DMF. If the peptide precipitates at any stage during this step, stop adding water and add a little more DMF until the peptide redissolves.
Purification
Greater than 85%
Recommended Storage
Store at -20°C for up to 1 year.
Usage
LS-E41666 is the immunizing peptide used to generate
LS-C358895. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Restrictions
For research use only. Intended for use by laboratory professionals.