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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PathPlus™ MCM2 Antibody aa1‑50 Polyclonal IF,IHC,Peptide‑ELISA,WB LS‑B7044

Catalog Number Size Price
LS-B7044-50 50 µl (1 mg/ml) $425 
Anti-MCM2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Immunofluorescence analysis of HepG2 cells, using MCM2 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293 cells, using MCM2 Antibody. The lane on the right is blocked with the synthesized peptide.
Anti-MCM2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Immunofluorescence analysis of HepG2 cells, using MCM2 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293 cells, using MCM2 Antibody. The lane on the right is blocked with the synthesized peptide.
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PathPlus™ MCM2 Antibody aa1‑50 Polyclonal IF,IHC,Peptide‑ELISA,WB LS‑B7044

Note: This antibody replaces LS-C118514
Antibody:
Rabbit Polyclonal to Human MCM2
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B7044-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human MCM2
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
MCM2 is a component of the MCM complex, a replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2 is required for entry into the S phase and for cell division. MCM2 is a useful marker in immunohistochemistry for characterizing a number of cancers. It is overexpressed in precancerous and cancerous cervical lesions and upregulation is associated with high-risk type HPV. In squamous cell and adenocarcinoma of the gallbladder, MCM2 positivity is associated with metastasis and poor prognosis. It has similar prognostic value in oral dysplasia and ovarian serous neoplasms, where expression increases with severity of tumor grade. In immunohistochemistry of normal tissue, MCM2 has nuclear positivity in proliferative cells throughout the body. Int J Clin Exp Pathol. 2015; 8(1): 875–880, PMID: 25755789; Mol Med Rep. 2016 Nov; 14(5): 4581–4592, PMID: 27748889; Applied Immunohistochemistry & Molecular Morphology. 2018 Aug. 26(7):509–513, DOI: 10.1097/PAI.0000000000000330; British Journal of Cancer. 2004. 90:1583–1590, PMID: 15083189
Target
Human MCM2
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human MCM2. (AA Range: 1-50)
Epitope
aa1-50
Specificity
MCM2 Antibody detects endogenous levels of total MCM2 protein.
Usage
Western Blot: Predicted MW 101 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About MCM2
P49736 NM_004526 NP_004517.2

Validation

Anti-MCM2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
PathPlus™ Validated
Anti-MCM2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-MCM2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

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Requested From: United States
Date Requested: 8/23/2019
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