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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B3160-0.1 0.1 mg $477 
KLK3 / PSA Antibody - Anti-KLK3 / PSA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

PathPlus™ Polyclonal Rabbit anti‑Human KLK3 / PSA Antibody (IHC) LS‑B3160

PathPlus™ Polyclonal Rabbit anti‑Human KLK3 / PSA Antibody (IHC) LS‑B3160

Note: This antibody replaces LS-C38409
Antibody:
Rabbit Polyclonal to Human KLK3 / PSA
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$477 
LS-B3160-0.1
0.1 mg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human KLK3 / PSA
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Prostate specific antigen (PSA), or Kallikrein-Related Peptidase 3 (KLK3), is a secreted protease produced in prostate glandular epithelium that is thought to function in the liquefaction of seminal fluid. PSA is a highly sensitive and specific diagnostic marker for recurrent prostate carcinoma after prostatectomy. It is also useful for confirming suspected prostatic origin in the setting of metastatic carcinoma. PSA is expected to have cytoplasmic staining in normal prostate tissue, periurethral and perianal glands, the bladder, the urethra and in seminal vesicles. PSA can also be found in tumors of the colon, liver, lung, parotid, adrenal, and ovary, and in metaplasias of the bladder and nodular hyperplasia or prostatitis. References: Oesterling, 1991; 1988; Stamey, 1987.
Target
Human KLK3 / PSA
Synonyms
KLK3 | APS | Gamma-seminoprotein | Kallikrein-3 | Kallikrein-related peptidase 3 | P-30 antigen | Prostate specific antigen | Prostate-specific antigen | PSA | Semenogelase | KLK2A1 | Seminin
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Ion exchange chromatography
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human prostate specific antigen.
Specificity
Recognizes human Prostate Specific Antigen, Total. No cross-reactivity with human albumin, AFP, CEA, ferritin, hCG, PAP, CA125, CA15-3 or CA19-9.
Usage
Immunohistochemistry: LS-B3160 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3160 was determined to be 5 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KLK3 / PSA
P07288 NM_001648 NP_001639.1

Validation

KLK3 / PSA Antibody - Anti-KLK3 / PSA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-KLK3 / PSA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-KLK3 / PSA antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

PathPlus™ KLK3 / PSA Antibody for IHC, ELISA LS-B3160 has an LSBio Rating of
Laboratory Validation Score (5)

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Requested From: United States
Date Requested: 7/10/2020