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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B8309-50 50 µg (0.5 mg/ml) $425 
ACPP / PAP Antibody - Anti-ACPP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
ACPP / PAP Antibody - ACPP antibody (0.3 ug/ml) staining of Human Prostate lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ACPP / PAP Antibody - Anti-ACPP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
ACPP / PAP Antibody - ACPP antibody (0.3 ug/ml) staining of Human Prostate lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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PathPlus™ Polyclonal Goat anti‑Human ACPP / PAP Antibody (aa200‑300, IHC, WB) LS‑B8309

PathPlus™ Polyclonal Goat anti‑Human ACPP / PAP Antibody (aa200‑300, IHC, WB) LS‑B8309

Note: This antibody replaces LS-C112967
Antibody:
Goat Polyclonal to Human ACPP / PAP
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B8309-50
50 µg
0.5 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Goat Polyclonal to Human ACPP / PAP
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
PSAP (ACPP, prostatic acid phosphatase) is a dephosphorylating enzyme produced in prostatic glandular epithelium. PSAP is a positive biomarker for hyperplastic prostate in immunohistochemistry and may be used to identify prostate origin in metastatic cancers. PSAP distinguishes prostate cancer (positive) from urothelial cancer, mesonephric remnant hyperplasia and clear cell tumors (generally negative). It is also positive in bladder and rectal carcinomas. Higher expression in tumors has been correlated with increasing tumor stage, although it is thought to be a tumor suppressant by means of vitamin D-associated slowing of prostate growth. Lower expression in normal tissue is therefore thought to be a risk factor for the development of cancer. In immunohistochemistry of normal tissue, antibodies to PSAP have cytoplasmic positivity in lysosomal granules in normal prostatic epithelial and ductal cells. References: Virchows Archiv: An International Journal of Pathology 2009-05; 454.5; 573-579, PMID: 19301031; Cancer Research 9/1/1992; 52.17; 4600-4607, PMID:1380886; The Journal of Steroid Biochemistry and Molecular Biology 2005-10; 97.43102; 37-46, PMID: 16076555; Biomolecules & Therapeutics 2013-01; 21.1; 43393, PMID: 24009853; Journal of Immunology (Baltimore, Md.: 1950) 12/15/2001; 167.12; 7150-7156, PMID: 11739538; The American Journal of Surgical Pathology 1991-08; 15.8; 785-790, PMID; 1712549; Appl Immunohistochem Mol Morphol. 2002 Sep;10(3):231-6, PMID: 12373149; Ann Diagn Pathol 2009;13:402, PMID: 19917478;
Target
Human ACPP / PAP
Synonyms
ACPP | ACP3 | 5-NT | 5-nucleotidase | Acid phosphatase, prostate | ACP-3 | Ecto-5-nucleotidase | PAP | Prostatic acid phosphatase | Thiamine monophosphatase | Prostatic acid phosphotase | TMPase
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide derived from the internal region of human ACPP.
Blocking Peptide
LS-E27071 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8309. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa200-300
Specificity
Human ACPP. This antibody is expected to recognize both isoforms (NP_001090.2; NP_001127666.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 50kDa band observed in Human Prostate lysates, with a calculated MW of 44.6kDa according to NP_001090.2. The recommended concentration is 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACPP / PAP
P15309 NM_001099 NP_001090.2

Validation

ACPP / PAP Antibody - Anti-ACPP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-ACPP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.
Anti-ACPP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.

LSBio Ratings

PathPlus™ ACPP / PAP Antibody (aa200-300) for IHC, WB/Western LS-B8309 has an LSBio Rating of
Laboratory Validation Score (5)

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Requested From: United States
Date Requested: 6/6/2020