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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16180-100 | 100 µl (0.05 mg/ml) | $485 |
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2 of 2
PathPlus™ Monoclonal Mouse anti‑Human TPSAB1 / Mast Cell Tryptase Antibody (clone 1.N.5, IHC, WB) LS‑B16180
PathPlus™ Monoclonal Mouse anti‑Human TPSAB1 / Mast Cell Tryptase Antibody (clone 1.N.5, IHC, WB) LS‑B16180
Note: This antibody replaces LS-C25452
Antibody:
TPSAB1 / Mast Cell Tryptase Mouse anti-Human Monoclonal (1.N.5) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Cat, Dog
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
TPSAB1 / Mast Cell Tryptase Mouse anti-Human Monoclonal (1.N.5) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Cat, Dog
Format:
Unconjugated, Unmodified
Specifications
Description
Tryptase alpha/beta 1 (TPSAB1) is one of two adjacent genes (TPSAB1, TPSB2) that code for mast cell tryptase, a serine protease. Mast cell tryptase is the predominant isoenzyme in mast cells. It is often used as a marker for mast cell activation, and high levels of tryptase can be an indicator of anaphylaxis, mastocytosis or the presence of eosinophilic leukemia. In immunohistochemistry, antibodies to mast cell tryptase stain secretory granules of mast cells.
References: J Biol Chem. 1999. 274 (6): 3355–62,PMID: 9920877; Nat Genet. 2016 Dec;48(12):1564-1569, PMID: 27749843; Proceedings of the National Academy of Sciences of the United States of America. 1990 May. 87 (10): 3811–5, PMID: 2187193; Cancer Research. 2017 Mar. 77 (6): 1261–1270, PMID: 28254862; Am. J. Clin. Path. 2015 Sept. 144 (3): 377–92, PMID 26276769; Anaesthesia. 2004 Jul;59(7):695-703, PMID: 15200544
Target
Human TPSAB1 / Mast Cell Tryptase
Synonyms
TPSAB1 | Alpha tryptase | Mast cell alpha II tryptase | Tryptase alpha-1 | Tryptase beta I | Tryptase-III | TPSB1 | Tryptase alpha II | TPS1 | TPS2 | Tryptase beta-1 | Tryptase-1 | Tryptase-I | Mast cell beta I tryptase | Tryptase alpha/beta 1 | Tryptase alpha/beta-1 | Tryptase beta 1 | Tryptase I
Host
Mouse
Reactivity
Human, Monkey, Cat, Dog
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
1.N.5
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Human mast cell tryptase isolated from purified human lung tissues.
Specificity
Reacts with Mast Cell Tryptase, both a and b isoforms. Species cross-reactivity: Human, monkey, canine and feline.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1 - 2 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1-2 ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 0.06-0.12 ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20min. Positive control: Tonsil.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 0.2% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TPSAB1 / Mast Cell Tryptase
Validation
Human Tonsil, Mast Cells: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil, Mast Cells: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas, Mast Cells: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas, Mast Cells: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ TPSAB1 / Mast Cell Tryptase Antibody (clone 1.N.5) for IHC, WB/Western LS-B16180 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin
Species:
Human
Applications:
IHC - Paraffin
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, ELISA
Species:
Human, Monkey, Cat, Dog
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Species:
Mouse
Applications:
Western blot, Immunoprecipitation
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
Date Requested: 4/29/2024
