Human SFN / Stratifin / 14-3-3 Sigma

Rabbit

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity chromatography

Unmodified

Recombinant full length protein of human 14-3-3 sigma

Recognizes endogenous levels of 14-3-3 sigma protein.

• IHC - Paraffin (5 µg/ml)
• ICC (1:50 - 1:200)
• Immunofluorescence (1:50 - 1:200)
• Western blot (1:500 - 1:2000)

Western blot analysis of 14-3-3 sigma expression in HT29 (A), MCF7 (B) whole cell lysates. Immunohistochemical analysis of 14-3-3 sigma staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Immunofluorescent analysis of 14-3-3 sigma staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P31947 NCBI: NM_006142 NP_006133.1