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| Catalog Number | Size | Price |
|---|---|---|
| LS-B10255-100 | 100 µl | $485 |
1 of 2
2 of 2
PathPlus™ Polyclonal Rabbit anti‑Human SF1 Antibody (aa65‑114, IHC, WB) LS‑B10255
PathPlus™ Polyclonal Rabbit anti‑Human SF1 Antibody (aa65‑114, IHC, WB) LS‑B10255
Note: This antibody replaces LS-C134961
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
Splicing Factor 1 (SF1, zinc finger protein 162) is a nuclear pre-mRNA splicing factor that is required for the early stages of spliceosome assembly. It plays a role in nuclear pre-mRNA retention and transcriptional repression. It is involved in testicular development, and in the development of Leydig and Sertoli cells. SF1 is widely expressed and is nuclear in localization. This protein is not to be confused with SRSF1, a proto-oncogene splicing factor also called Splicing Factor 1.
References: Mol Cell Biol 2008 28(9):3045; Cancer Res 2010 70(18):7264
Target
Human SF1
Synonyms
SF1 | D11S636 | MBBP | ZCCHC25 | Transcription factor ZFM1 | Zinc finger gene in MEN1 locus | Zinc finger protein 162 | BBP | ZNF162 | Splicing factor 1 | ZFM1
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide located between aa65-114 of human SF1 (Q15637, NP_004621). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Armadillo, Platypus, Xenopus, Stickleback, Zebrafish (100%); Drosophila (84%).
Epitope
aa65-114
Specificity
Human SF1
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- Western blot (1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SF1
Validation
Anti-SF1 antibody IHC staining of human lung alveoli, Type 2 pneumocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-SF1 antibody IHC staining of human lung alveoli, Type 2 pneumocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
PathPlus™ SF1 Antibody (aa65-114) for IHC, WB/Western LS-B10255 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
Species:
Human, Mouse, Rat, Bovine, Rabbit, Zebrafish
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/10/2024
Date Requested: 11/10/2024
