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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B16215-0.1 0.1 ml $250 
PSA-ACT Complex Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

PathPlus Cancer PathPlus™ PSA‑ACT Complex Antibody Monoclonal ELISA,IHC LS‑B16215

PathPlus Cancer PathPlus™ PSA‑ACT Complex Antibody Monoclonal ELISA,IHC LS‑B16215

Note: This antibody replaces LS-C757386
Antibody:
Mouse Monoclonal to Human PSA-ACT Complex
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$250 
LS-B16215-0.1
0.1 ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human PSA-ACT Complex
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Prostate Specific Antigen (PSA, KLK3) is a serine glycoprotein protease found in prostate tissue that is used in the diagnosis of prostate tumors, where levels of the protein are often elevated. Prostate Specific antigen is freely present in serum but may also be complexed with inhibitors such as alpha 1-antichymotrypsin (known as the PSA-ACT complex). When compared to free PSA, the PSA-ACT complex has higher levels in cancer than in benign tumors, and PSA-Act Complex antibodies may be more sensitive for cancer diagnosis than those that strictly target PSA. Cancer Res. 1991 Jan 1;51(1):222-6, PMID: 1703033; Prostate. 2013 Jan;73(2):219-26, PMID: 22806587; BJU Int. 2005 Apr;95(6):761-5, PMID: 15794778
Target
Human PSA-ACT Complex
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified. Also available Ready-to-Use.
Applications
  • IHC - Paraffin (1:100)
  • ELISA (1:100 - 1:200)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant Human PSA
Usage
Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual IHC Protocol: 1) Perform Heat-Induced Epitope Retrieval at pH 9 for 10 to 30 minutes. 2) Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System) 3) Dilute antibody 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4) Add secondary antibody and incubate for 20 to 30 minutes at room temperature. 5) Add DAB or Fast Red and incubate for 5 to 10 minutes at room temperature. 6) Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. Dehydrate and apply coverslip.
Presentation
Tris, pH 7.3-7.7, 1% BSA, <0.1% Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

PSA-ACT Complex Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
PathPlus™ Validated
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

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Requested From: United States
Date Requested: 10/19/2019