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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16215-0.05 | 0.05 ml | $460 |
PathPlus™ Monoclonal Mouse anti‑Human PSA‑ACT Complex Antibody (Concentrated, IHC) LS‑B16215
PathPlus™ Monoclonal Mouse anti‑Human PSA‑ACT Complex Antibody (Concentrated, IHC) LS‑B16215
Note: This antibody replaces LS-C757386
Antibody:
PSA-ACT Complex Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
PSA-ACT Complex Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Specifications
Description
Prostate Specific Antigen (PSA, KLK3) is a serine glycoprotein protease found in prostate tissue that is used in the diagnosis of prostate tumors, where levels of the protein are often elevated. Prostate Specific antigen is freely present in serum but may also be complexed with inhibitors such as alpha 1-antichymotrypsin (known as the PSA-ACT complex). When compared to free PSA, the PSA-ACT complex has higher levels in cancer than in benign tumors, and PSA-Act Complex antibodies may be more sensitive for cancer diagnosis than those that strictly target PSA.
References: Cancer Res. 1991 Jan 1;51(1):222-6, PMID: 1703033; Prostate. 2013 Jan;73(2):219-26, PMID: 22806587; BJU Int. 2005 Apr;95(6):761-5, PMID: 15794778
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Concentrated.
Also available Ready-to-Use.
Immunogen
Recombinant Human PSA
Applications
- IHC - Paraffin (1:100)
- ELISA (1:100 - 1:200)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual IHC Protocol: 1) Perform Heat-Induced Epitope Retrieval at pH 9 for 10 to 30 minutes. 2) Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System) 3) Dilute antibody 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4) Add secondary antibody and incubate for 20 to 30 minutes at room temperature. 5) Add DAB or Fast Red and incubate for 5 to 10 minutes at room temperature. 6) Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. Dehydrate and apply coverslip.
Presentation
Tris, pH 7.3-7.7, 1% BSA, <0.1% Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ PSA-ACT Complex Antibody (Concentrated) for IHC, ELISA LS-B16215 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
Western blot, Flow Cytometry
Species:
Human
Applications:
IHC - Paraffin, ELISA
Reactivity:
Human
Range:
0.469-30 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
Date Requested: 4/26/2024
