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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B6056-50 50 µl $425 
PDGFRA / PDGFR Alpha Antibody - Anti-PDGFRA antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

PathPlus™ Polyclonal Rabbit anti‑Human PDGFRA / PDGFR Alpha Antibody (aa1000‑C‑term, IHC, WB) LS‑B6056

PathPlus™ Polyclonal Rabbit anti‑Human PDGFRA / PDGFR Alpha Antibody (aa1000‑C‑term, IHC, WB) LS‑B6056

Note: This antibody replaces LS-C9640
Antibody:
PDGFRA / PDGFR Alpha Rabbit anti-Human Polyclonal (aa1000-C-term) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425
LS-B6056-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
PDGFRA / PDGFR Alpha Rabbit anti-Human Polyclonal (aa1000-C-term) Antibody
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
PDGFRA is a tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival, cell migration, chemotaxis in wound healing, and platelet activation and aggregation. Depending on the context, it promotes or inhibits cell proliferation and cell migration. It plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells and is required for normal skeleton development, the development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and the normal development of intestinal villi. In cancer, PDGFRA is overexpressed in gliomas, gastrointestinal stromal tumors (GIST), and ovarian, prostate, colon, lung, breast, bone and skin cancers. PDGFRA expression in ovarian cancer and chondrosarcoma is associated with shorter survival. In immunohistochemistry of normal tissue, PDGFRA is widely expressed throughout the body. References: J Cell Physiol. 1994 Feb; 158(2):381-9, PMID: 8106574; J Histochem Cytochem. 2016 Dec; 64(12): 785–810, PMID: 27837159; Am J Surg Pathol 2001;25:1520; Sci Rep. 2015 Dec 7;5:17948, PMID: 26639755
Target
Human PDGFRA / PDGFR Alpha
Synonyms
PDGFRA | CD140A | CD140a antigen | PDGFalphaR | PDGFR2 | PDGFRalpha | AlphaPDGFR | RHEPDGFRA | PDGF receptor alpha | PDGFR-2 | PDGFRA/BCR fusion | APDGFR | Pdgf alpha-receptor | PDGF-R-alpha | PDGFR-alpha
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to C-Terminus of human PDGF Receptor alpha.
Epitope
aa1000-C-term
Specificity
Recognizes human PDGF Receptor Type A, doublet, Mr 150/170kD. Species Crossreactivity: mouse. Species Sequence Homology: frog, chicken and zebra fish.
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:250 - 1:1000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:250-1:1000 dilution detected PDGF Receptor a doublet (150/170kD) in 3T3/NIH cell lysate. 3T3/NIH cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PDGF Receptor a (1:500 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5-10 ul immunoprecipitates PDGF Receptor a from 500 ug of 3T3/NIH RIPA lysate.
Presentation
0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, 0.05% Sodium Azide
Storage
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 1 year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDGFRA / PDGFR Alpha
P16234 NM_006206 NP_006197.1

Validation

PDGFRA / PDGFR Alpha Antibody - Anti-PDGFRA antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-PDGFRA antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-PDGFRA antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

LSBio Ratings

PathPlus™ PDGFRA / PDGFR Alpha Antibody (aa1000-C-term) for IHC, WB/Western, IP LS-B6056 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (5)

Publications (2)

A genome-wide association study for corneal curvature identifies the platelet-derived growth factor receptor alpha gene as a quantitative trait locus for eye size in white Europeans. Guggenheim JA, McMahon G, Kemp JP, Akhtar S, St Pourcain B, Northstone K, Ring SM, Evans DM, Smith GD, Timpson NJ, Williams C. Molecular vision. 2013 19:243-53.

Imatinib mesylate plus hydroxyurea chemotherapy for cerebellar meningioma in a Belgian Malinois dog. Jung HW, Lee HC, Kim JH, Jang HM, Moon JH, Sur JH, Ha J, Jung DI. The Journal of veterinary medical science. 2014 76:1545-8. (IHC; Canine)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/29/2020