Menu
Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C18692-100 100 µg (1 mg/ml) $445 
LAG3 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

PathPlus™ LAG3 Antibody aa70‑99 Monoclonal ICC,IHC,IP,WB LS‑C18692

PathPlus™ LAG3 Antibody aa70‑99 Monoclonal ICC,IHC,IP,WB LS‑C18692

Antibody:
Mouse Monoclonal to Human LAG3
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$445 
LS-C18692-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human LAG3
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Lymphocyte activation gene-3 (LAG3, CD223) is membranous co-inhibitory cell surface receptor expressed on activated CD4 and CD8 T-cells that controls immune responses and acts as an immune checkpoint receptor. It is expressed on activated T-cells, natural killer cells, and plasmacytoid dendritic calls. LAG3 negatively regulates cell proliferation, activation, cytokine production and Treg cell mediated control of homeostasis of T cells. Lag-3 is expressed on exhausted effector tumor infiltrating lymphocytes and blockade has been demonstrated to improve anti-tumor CD8 T cell responses. It is the target of drug development to treat cancer, chronic viral infection and autoimmune disorders. Immunity 2016, 44:989
Target
Human LAG3
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
17B4
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • IHC - Frozen (1:150)
  • ICC
  • Western blot (5 µg/ml)
  • Immunoprecipitation (10 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-terminal D1 domain of human LAG-3 (lymphocyte activation gene-3). Percent identity by BLAST analysis: Human (100%); Gorilla (93%); Gibbon (90%); Monkey (83%).
Epitope
aa70-99
Specificity
Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of human LAG-3.
Presentation
Supplied as a liquid in PBS containing 10% glycerol and 0.02% sodium azide.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LAG3
P18627 NM_002286 P18627

Validation

LAG3 Antibody - Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Publications (3)

Differential Expression of Immune-Regulatory Genes Associated with PD-L1 Display in Melanoma: Implications for PD-1 Pathway Blockade. Taube JM, Young GD, McMiller TL, Chen S, Salas JT, Pritchard TS, Xu H, Meeker AK, Fan J, Cheadle C, Berger AE, Pardoll DM, Topalian SL. Clinical cancer research : an official journal of the American Association for Cancer Research. 2015 21:3969-76. (IHC; Human)

Analysis of Immune Signatures in Longitudinal Tumor Samples Yields Insight into Biomarkers of Response and Mechanisms of Resistance to Immune Checkpoint Blockade. Chen PL, Roh W, Reuben A, Cooper ZA, Spencer CN, Prieto PA, Miller JP, Bassett RL, Gopalakrishnan V, Wani K, De Macedo MP, Austin-Breneman JL, Jiang H, Chang Q, Reddy SM, Chen WS, Tetzlaff MT, Broaddus RJ, Davies MA, Gershenwald JE, Haydu L, Lazar AJ, Patel SP, Hwu P, Hwu WJ, Diab A, Glitza IC, Woodman SE, Vence LM, Wistuba II, Amaria RN, Kwong LN, Prieto V, Davis RE, Ma W, Overwijk WW, Sharpe AH, Hu J, Futreal PA, Blando J, Sharma P, Allison JP, Chin L, Wargo JA. Cancer discovery. 2016 6:827-37. (IHC; Human)

The upregulation of LAG-3 on T cells defines a subpopulation with functional exhaustion and correlates with disease progression in HIV-infected subjects. Tian X, Zhang A, Qiu C, Wang W, Yang Y, Qiu C, Liu A, Zhu L, Yuan S, Hu H, Wang W, Wei Q, Zhang X, Xu J. Journal of immunology (Baltimore, Md. : 1950). 2015 194:3873-82. (IHC; Human)

Customer Reviews (0)


Requested From: United States
Date Requested: 11/20/2019