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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16328-0.05 | 0.05 ml | $460 |
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PathPlus™ Monoclonal Mouse anti‑Human Hairy Cell Leukemia Antibody (Concentrated, IHC) LS‑B16328
PathPlus™ Monoclonal Mouse anti‑Human Hairy Cell Leukemia Antibody (Concentrated, IHC) LS‑B16328
Note: This antibody replaces LS-C759829
Antibody:
Hairy Cell Leukemia Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
Hairy Cell Leukemia Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Specifications
Description
Hairy Cell Leukemia antibodies recognize a membrane antigen on sub-populations of B-lymphocytes and were originally developed against a B-lymphoma cell line (al Saati et al, 1989). Antibodies to hairy cell leukemia stain B-cells in the follicular mantle zone and nearly all hairy cell leukemia. They are useful for differentiating hairy cell leukemia from B-cell chronic lymphocytic leukemia. They also stain follicular center-cell lymphomas, some high grade centroblastic and immunoblastic B-cell lymphomas, and splenic margin zone lymphomas with villous lymphocytes.
References: al Saati et al. Blood. 1989 Nov 15;74(7):2476-85, PMID: 2679917; Leuk Res. 1996 Nov-Dec;20(11-12):909-13, PMID: 9009248; Am J Surg Pathol. 2007;31:438, PMID:17325486; American Journal of Clinical Pathology. 2011 Sep. 136(3):390–399, DOI: 10.1309/AJCP5GE1PSBMBZTW;
Target
Human Hairy Cell Leukemia
Synonyms
HCL | DBA.44 | DBA44
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgM
Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Concentrated.
Also available Ready-to-Use.
Immunogen
Large cell lymphoma cells.
Applications
- IHC (1:100 - 1:200)
- IHC - Paraffin (1:50)
- ELISA (1:100 - 1:200)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual Use: 1. Pretreatment: Perform heat-induced epitope retrieval (HIER) at pH 9 for 10 to 30 minutes. 2. Peroxide Block: Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System.) 3. Primary Antibody: Dilute antibody at 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4. Secondary Antibody: Incubate for 20 to 30 minutes at room temperature. 5. Substrate Development: Incubate DAB or Fast Red for 5 to 10 minutes at room temperature. 6. Counterstain: Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. 7. Dehydrate and apply coverslip.
Presentation
Tris, pH 7.3-7.7, 1% BSA, <0.1% Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
PathPlus™ Hairy Cell Leukemia Antibody (Concentrated) for IHC, ELISA LS-B16328 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC - Paraffin
Species:
Human
Applications:
IHC - Paraffin
Reactivity:
Human
Range:
3.12-200 pg/ml
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/3/2024
Date Requested: 5/3/2024
