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Catalog Number Size Price
LS-B16245-100 100 µg (200 µg/ml) $485 
FOXA1 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
FOXA1 Antibody - Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
FOXA1 Antibody - SDS-PAGE Analysis Purified FOXA1 Mouse Recombinant Monoclonal Antibody (rFOXA1/1515). Confirmation of Purity and Integrity of Antibody.
FOXA1 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
FOXA1 Antibody - Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
FOXA1 Antibody - SDS-PAGE Analysis Purified FOXA1 Mouse Recombinant Monoclonal Antibody (rFOXA1/1515). Confirmation of Purity and Integrity of Antibody.
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PathPlus™ Recombinant Monoclonal Mouse anti‑Human FOXA1 Antibody (clone rFOXA1/1515, aa372‑472, IHC) LS‑B16245

PathPlus™ Recombinant Monoclonal Mouse anti‑Human FOXA1 Antibody (clone rFOXA1/1515, aa372‑472, IHC) LS‑B16245

Note: This antibody replaces LS-C751174
Antibody:
FOXA1 Mouse anti-Human Recombinant Monoclonal (aa372-472) (rFOXA1/1515) Antibody
Application:
IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485
LS-B16245-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
FOXA1 Mouse anti-Human Recombinant Monoclonal (aa372-472) (rFOXA1/1515) Antibody
Application:
IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
FOXA1 (forkhead box A1, hepatocyte neuclear factor 3a, HNF3A) is a forkhead box domain transcription factor and nuclear steroid receptor involved in mediating sex hormone-dependent signaling in the differentiation and development of tissues such as the pancreas, liver, breast and prostate. Expression of FOXA1 has been associated with a good prognosis in hormone receptor positive breast cancer. It is a useful marker for luminal A type breast cancers, as it is often expressed in luminal subtypes independent of ER expression and it does not see expression in basal subtypes. In ER-negative tumors, a lack of FOXA1 expression is associated with worse prognosis, as loss of FOXA1 is associated with the consequential expression of metastases-prone, basal-specific proteins that it normally acts to directly repress. FOXA1 is expected to show nuclear expression in tissues such as prostate, breast, urothelium, the GI tract and respiratory epithelium. References: EMBO J. 2011 30(19):3885; Bernardo, 2013; Algergaria, 2001; Gong, 2014; Expert Rev Mol Med 2009 5(11):e8; Breast Cancer Res 2009 11(3):R40; Mol Endocrinol 2010 24(8):1594; Ann Surg Oncol 2012 19(4):1145
Target
Human FOXA1
Synonyms
FOXA1 | Forkhead box A1 | Forkhead box protein A1 | HNF-3-alpha | HNF-3A | TCF-3A | TCF3A | HNF3A | Transcription factor 3A
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Recombinant Monoclonal
Clone
rFOXA1/1515
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified. Also available Azide-free, Carrier-free.
Immunogen
Recombinant human FOXA1 protein fragment (around aa372-472). Antigen Molecular Weight: 49 kDa
Epitope
aa372-472
Specificity
The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (alpha and beta), HNF-3 (alpha, beta and gamma), HNF-4 (alpha and gamma), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.
Applications
  • IHC - Paraffin (1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistology (Formalin-fixed - 1-2 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.
Presentation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Storage
Store at 2°C to 8°C for up to 2 years.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FOXA1
P55317 NM_004496 NP_004487.2

Validation

FOXA1 Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

FOXA1 Antibody - Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ FOXA1 Antibody (aa372-472, clone rFOXA1/1515) for IHC LS-B16245 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 9/24/2021