Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-A6603-50 50 µg (1 mg/ml) $460 
CRFR1 / CRHR1 Antibody - Anti-CRF1 antibody IHC of human pars intermedia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

PathPlus™ Polyclonal Rabbit anti‑Human CRFR1 / CRHR1 Antibody (N‑Terminus, IHC) LS‑A6603

PathPlus™ Polyclonal Rabbit anti‑Human CRFR1 / CRHR1 Antibody (N‑Terminus, IHC) LS‑A6603

Antibody:
CRFR1 / CRHR1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-A6603-50

Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CRFR1 / CRHR1 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse
Format:
Unconjugated, Unmodified

Specifications

Description
Corticotropin releasing hormone receptor 1 (CRHR1) is a G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). CRHR1 has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. CRHR1 promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels and inhibits the activity of the calcium channel CACNA1H. CRHR1 is required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress and plays a role in the response to anxiogenic stimuli. References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804
Target
Human CRFR1 / CRHR1
Synonyms
CRHR1 | CRF-R-1 | CRFR | CRHR1f | Crf receptor 1 | CRH-R1 | CRF-R | CRF1 | CRFR-1 | Crf receptor type 1 | Crf receptor type 1 | Crf type 1 | CRF-R1 | Crf1 receptor | CRFR1 | CRH-R-1 | CRH-R1h | CRHR | CRHR1L
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Dog, Hamster, Horse (tested or 100% immunogen sequence identity)
Predicted
Bat, Bovine, Pig (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 15 amino acid peptide from N-Terminus extracellular domain of human CRF1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Dog, Hamster, Panda, Horse (100%); Bat, Bovine, Pig, Opossum (93%); Elephant (87%); Sheep, Lizard (80%).
Epitope
N-Terminus
Specificity
Human CRF1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except CRHR2 (73%).
Applications
  • IHC
  • IHC - Paraffin (15 - 20 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CRFR1 / CRHR1
P34998 NM_004382 P34998

Validation

CRFR1 / CRHR1 Antibody - Anti-CRF1 antibody IHC of human pars intermedia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRF1 antibody IHC of human pars intermedia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CRF1 antibody IHC of human pars intermedia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

PathPlus™ CRFR1 / CRHR1 Antibody (N-Terminus) for IHC LS-A6603 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)

Publications (1)

Does the corticotropin-releasing hormone system play a role in the pathogenesis of lichen planus?. Cemil BC, Yilmazer D, Atas H, Cengiz FP, Canpolat F, Alper M, Gonul M. Postepy dermatologii i alergologii. 2017 August;34:322-327. (Human)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 7/18/2024