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Catalog Number Size Price
LS-B1956-50 50 µg (0.5 mg/ml) $440 
CD40 Antibody - Anti-CD40 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CD40 Antibody - Human peripheral blood lymphocytes stained with purified HB14, followed by anti-mouse IgGs FITC
CD40 Antibody - Anti-CD40 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
CD40 Antibody - Human peripheral blood lymphocytes stained with purified HB14, followed by anti-mouse IgGs FITC
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PathPlus™ Monoclonal Mouse anti‑Human CD40 Antibody (clone HB14, IHC) LS‑B1956

PathPlus™ Monoclonal Mouse anti‑Human CD40 Antibody (clone HB14, IHC) LS‑B1956

Note: This antibody replaces LS-C40872
Antibody:
CD40 Mouse anti-Human Monoclonal (HB14) Antibody
Application:
IHC, IHC-P, Flo
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-B1956-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD40 Mouse anti-Human Monoclonal (HB14) Antibody
Application:
IHC, IHC-P, Flo
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
CD40 is a costimulatory protein of the TNF-receptor superfamily that is expressed on the membrane of antigen presenting cells and is involved in their activation. It is a vital regulator of numerous inflammatory and immune responses and functions in germinal center formation and memory B cell development. It is also required for amyloid-beta-induced microglial activation and is thought to be involved in the pathogenesis of Alzheimer’s disease. Absence of CD40 leads to Hyper-IgM syndrome type 3. In immunohistochemistry of normal tissue, CD40 has membranous and extracellular positivity in a wide range of cell types, including: B cells, dendritic cells, mast cells, monocytes, platelets, macrophages, endothelial cells, epithelial cells, fibroblasts, keratinocytes, Langerhans cells, nevus cells, pancreatic islet cells, prostatic acini, astrocytes and microglial cells. References: Immunology. 16: 111–35, PMID: 9597126; CNS Neurol Disord Drug Targets. 2010 Apr;9(2):149-55, PMID: 20205645; J Neuroinflammation. 2006 Feb 24;3:3, PMID: 16504112; Diabetologia. 2005 Feb;48(2):268-76, PMID: 15690148
Target
Human CD40
Synonyms
CD40 | B-cell surface antigen CD40 | B cell-associated molecule | B cell surface antigen CD40 | CD40 antigen | CD40 type II isoform | p50 | Bp50 | CD40L receptor | CDW40 | TNFRSF5
Host
Mouse
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
HB14
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Human B cell line.
Specificity
Recognizes human CD40.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Flow Cytometry (0.5 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B1956 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1956 was determined to be 10 ug/ml.
Presentation
PBS, pH 7.2, 0.09% Sodium Azide
Storage
Store at 4°C. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD40
P25942 NM_001250 NP_001241.1

Validation

CD40 Antibody - Anti-CD40 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CD40 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CD40 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

PathPlus™ CD40 Antibody (clone HB14) for IHC, Flow LS-B1956 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (5)

Publications (2)

Leishmania major infection activates NF-kappaB and interferon regulatory factors 1 and 8 in human dendritic cells. Jayakumar A, Donovan MJ, Tripathi V, Ramalho-Ortigao M, McDowell MA. Infection and immunity. 2008 76:2138-48.

Characterization of a rare IL-10-competent B-cell subset in humans that parallels mouse regulatory B10 cells. Iwata Y, Matsushita T, Horikawa M, Dilillo DJ, Yanaba K, Venturi GM, Szabolcs PM, Bernstein SH, Magro CM, Williams AD, Hall RP, St Clair EW, Tedder TF. Blood. 2011 117:530-41.

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 8/18/2022