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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B8447-50 50 µl (0.2 mg/ml) $485 
SMARCA4 / BRG1 Antibody - Anti-BRG1 / SMARCA4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

PathPlus™ Polyclonal Rabbit anti‑Human SMARCA4 / BRG1 Antibody (aa75‑125, IHC, WB) LS‑B8447

PathPlus™ Polyclonal Rabbit anti‑Human SMARCA4 / BRG1 Antibody (aa75‑125, IHC, WB) LS‑B8447

Note: This antibody replaces LS-C89131
Antibody:
Rabbit Polyclonal to Human SMARCA4 / BRG1
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485 
LS-B8447-50
50 µl
0.2 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human SMARCA4 / BRG1
Application:
IHC, IHC-P, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
SMARCA4 (Transcription activator BRG1) is a SWI/SNF protein that activates and represses transcription. It is involved in chromatin remodeling and is required for activation of genes repressed by chromatin. It is involved in embryogenesis, spermatogenesis and tissue development, including proper smooth muscle formation and synapse development. It is also involved in oligodendrocyte differentiation. Loss of SMARCA4 results in developmental defects such as incomplete or dysfunctional gastrointestinal tracts, open ductus arteriosus of the heart, as well as severe neurological dysfunction. Furthermore, inherited mutations in SMARCA4 have been linked with Coffin-Siris Syndrome in humans, characterized by developmental delays and intellectual disability. In cancer, SMARCA4 is the most commonly mutated chromatin remodeling ATPase, with mutations found in a broad spectrum of tumors such as lung, adrenal and pancreatic cancers as well as medulloblastomas. In immunohistochemistry, SMARCA4 has nuclear positivity in all tissues throughout the body. References: Molecular and Cellular Biology. 31 (13): 2618–31, PMID: 21518954; Development. 139 (6): 1133–40, PMID: 22318225; Proceedings of the National Academy of Sciences of the United States of America. 109 (5): E252–9, PMID: 22233809. PLoS One. 8 (1): e55119, PMID: 23355908; Cold Spring Harbor Perspectives in Medicine. 6 (8): a026930, PMID: 27413115; Mol Cell Biol. 2016 Jan 1; 36(1): 70–83, PMID: 26459759; Cell. 2013 Jan 17;152(1-2):248-61, PMID: 23332759; Front. Mol. Neurosci. 2017. DOI: 10.3389/fnmol.2017.00243
Target
Human SMARCA4 / BRG1
Synonyms
SMARCA4 | ATP-dependent helicase SMARCA4 | BAF190 | BRG1-associated factor 190A | Brahma protein-like 1 | BRG1 | BRM/SWI2-related gene 1 | HSNF2b | MRD16 | Protein BRG-1 | SNF2-like 4 | SNF2LB | SNF2-BETA | SNF2B | SWI2 | Nuclear protein GRB1 | SNF2 | SNF2L4 | Transcription activator BRG1 | Homeotic gene regulator | Protein brahma homolog 1 | RTPS2 | Sucrose nonfermenting-like 4
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide corresponding to amino acid residues 75 and 125 of human BRM/SWI2-Related Gene 1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of chromatin, Subfamily A, member 4) using the numbering given in entry NP_003063.2 (GeneID 6597).
Epitope
aa75-125
Specificity
Species cross-reactivity: Human and mouse. Other species have not been tested.
Usage
Suitable for use in Western Blot, Immunohistochemistry with FFPE tissues and Immunoprecipitation. Immunoprecipitation: 2-5 ug/mg lysate. Western Blot: 1:2000-1:10000.
Presentation
TBS, pH 7.2, 0.09% Sodium Azide, 0.1% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SMARCA4 / BRG1
P51532 NM_003072 NP_003063.2

Validation

SMARCA4 / BRG1 Antibody - Anti-BRG1 / SMARCA4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-BRG1 / SMARCA4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-BRG1 / SMARCA4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

LSBio Ratings

PathPlus™ SMARCA4 / BRG1 Antibody (aa75-125) for IHC, WB/Western, IP LS-B8447 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)

Publications (1)

Transcription factor Foxp3 and its protein partners form a complex regulatory network. Rudra D, deRoos P, Chaudhry A, Niec RE, Arvey A, Samstein RM, Leslie C, Shaffer SA, Goodlett DR, Rudensky AY. Nature immunology. 2012 Oct;13:1010-9. (ICC, WB; Human)

Customer Reviews (0)


Requested From: United States
Date Requested: 6/4/2020