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Immunohistochemistry Services

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-A2118-50 50 µg (1 mg/ml) $425 
HTR5A / 5-HT5A Receptor Antibody - Anti-5HT5A Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

PathPlus™ Polyclonal Rabbit anti‑Human HTR5A / 5‑HT5A Receptor Antibody (N‑Terminus, IHC) LS‑A2118

PathPlus™ Polyclonal Rabbit anti‑Human HTR5A / 5‑HT5A Receptor Antibody (N‑Terminus, IHC) LS‑A2118

Note: This antibody replaces LS-C91327, LS-C42662
Antibody:
Rabbit Polyclonal to Human HTR5A / 5-HT5A Receptor
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A2118-50
50 µg
1 mg/ml

Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human HTR5A / 5-HT5A Receptor
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
HTR5A (5-HT5a, 5-hydroxytryptamine serotonin receptor 5A) is a multi-pass membrane receptor for serotonin that negatively regulates adenylate cyclase. It is relevant to psychiatric diseases that involve serotonin and may be important for mood regulation and disorder. Furthermore, both blocking and promoting expression of HTR5A impact short- and long-term memory and it is considered to be a potential therapy target for schizophrenia and memory deficit disorders. HTR5A agonists include valerenic acid, olanzapine and lysergic acid diethylamide, while antagonists include risperidone, latrepirdine, AS-2674723, AS-2030680, ASP-5736 and SB-699,551. In immunohistochemistry, HTR5A has membranous positivity in the cerebellum, cortex, hippocampus, nucleus accumbens, hypothalamus and amygdala in the brain, and is also positive at low levels in the gastrointestinal tract, thyroid, lung, pancreas, skin, appendix, breast and adrenal gland. References: J Pharmacol Sci. 2015 Mar;127(3):362-9, PMID: 25837935; Mol Neurodegener. 2008 Oct 21;3:15, PMID: 18939977; Brain Research. Molecular Brain Research. 138 (2): 191–7, PMID: 15921820; European Journal of Pharmacology. 361 (2–3): 299–309, PMID: 9865521; J Neurosci. 2012 Apr 25;32(17):5804-9, PMID: 22539842;
Target
Human HTR5A / 5-HT5A Receptor
Synonyms
HTR5A | 5-HT-5 | 5-HT-5A | 5-HT5A | 5-HT5a receptor | 5-hydroxytryptamine5a | Serotonin 5-HT5a receptor | Serotonin 5a receptor | Serotonin receptor 5A
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 16 amino acid peptide from N-Terminus of human 5HT5A Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey (94%); Marmoset (88%).
Blocking Peptide
LS-E28504 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A2118. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human 5HT5A Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except TRIOBP (44%), SNCG (44%).
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 5 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HTR5A / 5-HT5A Receptor
P47898 NM_024012 NP_076917.1

Validation

HTR5A / 5-HT5A Receptor Antibody - Anti-5HT5A Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-5HT5A Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-5HT5A Receptor antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

PathPlus™ HTR5A / 5-HT5A Receptor Antibody (N-Terminus) for IHC, ELISA LS-A2118 has an LSBio Rating of
Laboratory Validation Score (5)

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Requested From: United States
Date Requested: 5/31/2020