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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B4244-50 50 µg (1 mg/ml) $425 
HNE / Neutrophil Elastase Antibody - Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
HNE / Neutrophil Elastase Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
HNE / Neutrophil Elastase Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
HNE / Neutrophil Elastase Antibody - Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
HNE / Neutrophil Elastase Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
HNE / Neutrophil Elastase Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
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PathPlus™ Polyclonal Sheep anti‑Human HNE / Neutrophil Elastase Antibody (IHC) LS‑B4244

PathPlus™ Polyclonal Sheep anti‑Human HNE / Neutrophil Elastase Antibody (IHC) LS‑B4244

Note: This antibody replaces LS-C23010
Antibody:
Sheep Polyclonal to Human HNE / Neutrophil Elastase
Application:
IHC-P, ELISA, IE
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B4244-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Sheep Polyclonal to Human HNE / Neutrophil Elastase
Application:
IHC-P, ELISA, IE
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ELANE (HNE, Neutrophil Elastase) is a serine protease that hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. It is thought to participate in degenerative and inflammatory diseases via its proteolysis of collagen-IV and elastin of the extracellular matrix. ELANE mutations are correlated with cyclic neutropenia and severe congenital neutropenia (SCN). In immunohistochemistry, ELANE has cytoplasmic positivity in neutrophils and bone marrow cells. References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804; BMC Hematol 15, 2 (2015), DOI:10.1186/s12878-015-0020-x
Target
Human HNE / Neutrophil Elastase
Synonyms
ELANE | Bone marrow serine protease | ELA2 | Elastase 2, neutrophil | Elastase-2 | HLE | Medullasin | Granulocyte-derived elastase | Neutrophil elastase | NE | Human leukocyte elastase | PMN elastase | PMN-E | SCN1 | Polymorphonuclear elastase | Elastase, neutrophil expressed | GE | Leukocyte elastase | Lysosomal elastase | HNE
Host
Sheep
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC - Paraffin (5 µg/ml)
  • ELISA
  • Immunoelectrophoresis
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Neutrophil elastase (active-site blocked) prepared from human sputum.
Specificity
Specific for elastase.
Usage
Suitable for use in ELISA, IHC with FFPE tissues and Immunoelectrophoresis.
Presentation
10 mM HEPES, pH 7.4, 0.15 M NaCl, 50% Glycerol
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HNE / Neutrophil Elastase
P08246 NM_001972 NP_001963.1

Validation

HNE / Neutrophil Elastase Antibody - Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE)

HNE / Neutrophil Elastase Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

HNE / Neutrophil Elastase Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ HNE / Neutrophil Elastase Antibody for IHC, ELISA LS-B4244 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)

Publications (1)

Intrarenal Toll-like receptor 4 and Toll-like receptor 2 expression correlates with injury in antineutrophil cytoplasmic antibody-associated vasculitis. O'Sullivan KM, Ford SL, Longano A, Kitching AR, Holdsworth SR. American journal of physiology. Renal physiology. 2018 November;315:F1283-F1294.

Customer Reviews (0)


Requested From: United States
Date Requested: 7/14/2020