Menu
Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-A9704-50 50 µg (1 mg/ml) $425 
TLR4 Antibody - Anti-TLR4 antibody IHC of human placenta, decidua. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

PathPlus™ TLR4 Antibody Internal Polyclonal IHC LS‑A9704

PathPlus™ TLR4 Antibody Internal Polyclonal IHC LS‑A9704

Antibody:
Rabbit Polyclonal to Human TLR4
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-A9704-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human TLR4
Application:
IHC, IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
TLR4 (Toll-like receptor 4) is a transmembrane protein involved in the immune response, inflammation, and cytokine secretion. TLR4 activates the NF-kappa-B (NFKB) and po-Interleukin-1 beta (IL1B) pathways in the brain and is thus relevant to neuro-inflammation and pathological neurodegenerative conditions. It has been found to positively regulate the proliferation of Schwann cells in the peripheral nervous system, it is upregulated in injured nerves, and it is involved in inflammation in brain injury. TLR4 is also expressed in astrocytes and neurons as part of the immune response to bacterial lipopolysaccharide (LPS). Likewise, mutations in TLR4 lead to susceptibility to infections by gram-negative bacteria. Additionally, TLR4 is expressed on many cancer cells, where it may increase proliferative capacity and resistance to therapies. In immunohistochemistry of normal tissue, TLR4 has highest cytoplasmic positivity in immune cells, in the placenta, and in microglia and neurons in the brain. Archives of Internal Medicine. 162 (9): 1028–32,PMID: 11996613; Cytokine. 89: 127–135, PMID: 26854213; Sci Rep. 2018; 8: 11179, PMID: 30046125; Front Immunol. 2019 May 10;10:1000, PMID: 31134076; Front Immunol. 2019 May 10;10:1000, PMID: 31134076
Target
Human TLR4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic 15 amino acid peptide from internal region of human TLR4. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon (100%); Baboon, Monkey (93%); Elephant, Cat (87%); Marmoset, Panda, Dog, Horse (80%).
Blocking Peptide
LS-E29740 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A9704. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Specificity
Human TLR4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TLR4
O00206 NM_138554 O00206

Validation

TLR4 Antibody - Anti-TLR4 antibody IHC of human placenta, decidua. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TLR4 antibody IHC of human placenta, decidua. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TLR4 antibody IHC of human placenta, decidua. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Publications (0)

Customer Reviews (0)


Requested From: United States
Date Requested: 12/15/2019