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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B2070-50 50 µg (1 mg/ml) $425 
TLR4 Antibody - Anti-TLR4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TLR4 Antibody - Western blot of TLR4 using antibody at 2 ug/ml on (A) human intestine and 6 ug/ml on (B) mouse intestine and C) rat intestine lysate.
TLR4 Antibody - Flow analysis of cell surface TLR4 in mouse splenocytes using 0.5 ug/10^6 cells of antibody.  Shaded histogram represents cells without anti-TLR4 antibody; green represents isotype control (BD Pharmingen); red represents anti-TLR4 antibody.
TLR4 Antibody - Anti-TLR4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
TLR4 Antibody - Western blot of TLR4 using antibody at 2 ug/ml on (A) human intestine and 6 ug/ml on (B) mouse intestine and C) rat intestine lysate.
TLR4 Antibody - Flow analysis of cell surface TLR4 in mouse splenocytes using 0.5 ug/10^6 cells of antibody.  Shaded histogram represents cells without anti-TLR4 antibody; green represents isotype control (BD Pharmingen); red represents anti-TLR4 antibody.
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PathPlus™ TLR4 Antibody aa100‑200 Monoclonal Flo,IHC,WB LS‑B2070

PathPlus™ TLR4 Antibody aa100‑200 Monoclonal Flo,IHC,WB LS‑B2070

Note: This antibody replaces LS-C547
Antibody:
Mouse Monoclonal to Human TLR4
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2070-50
50 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human TLR4
Application:
IHC, IHC-P, WB, Flo
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
TLR4 (Toll-like receptor 4) is a transmembrane protein involved in the immune response, inflammation, and cytokine secretion. TLR4 activates the NF-kappa-B (NFKB) and po-Interleukin-1 beta (IL1B) pathways in the brain and is thus relevant to neuro-inflammation and pathological neurodegenerative conditions. It has been found to positively regulate the proliferation of Schwann cells in the peripheral nervous system, it is upregulated in injured nerves, and it is involved in inflammation in brain injury. TLR4 is also expressed in astrocytes and neurons as part of the immune response to bacterial lipopolysaccharide (LPS). Likewise, mutations in TLR4 lead to susceptibility to infections by gram-negative bacteria. Additionally, TLR4 is expressed on many cancer cells, where it may increase proliferative capacity and resistance to therapies. In immunohistochemistry of normal tissue, TLR4 has highest cytoplasmic positivity in immune cells, in the placenta, and in microglia and neurons in the brain. Archives of Internal Medicine. 162 (9): 1028–32,PMID: 11996613; Cytokine. 89: 127–135, PMID: 26854213; Sci Rep. 2018; 8: 11179, PMID: 30046125; Front Immunol. 2019 May 10;10:1000, PMID: 31134076; Front Immunol. 2019 May 10;10:1000, PMID: 31134076
Target
Human TLR4
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
76B357.1
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human TLR4.
Epitope
aa100-200
Specificity
The amino acid sequence used has 100% identity in human, chimp, baboon, Chinese hamster, and rat; 93% identity in bovine and pig; 85% identity in sheep and 78% identity in cat, horse and mouse.
Usage
Immunohistochemistry: LS-B2070 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2070 was determined to be 5 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TLR4
O00206 NM_138554 O00206

Validation

TLR4 Antibody - Anti-TLR4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-TLR4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Publications (1)

Tissue expression of Toll-like receptors 2 and 4 in sporadic human colorectal cancer. Nihon-Yanagi Y, Terai K, Murano T, Matsumoto T, Okazumi S. Cancer immunology, immunotherapy : CII. 2013 Jan;61:71-7.

Customer Reviews (0)


Requested From: United States
Date Requested: 12/7/2019