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| Catalog Number | Size | Price |
|---|---|---|
| LS-N55443-1 | 1 Each | $216 |
Human H6PD / G6PDH Expression-Ready ORF Clone (pCMV3-C-His) LS-N55443
Human H6PD / G6PDH Expression-Ready ORF Clone (pCMV3-C-His) LS-N55443
Description:
Human H6PD / G6PDH Expression-Ready ORF Clone in Mammalian Expression Vector pCMV3-C-His, with C-Terminal His tag. Approximately 10 ug per unit.
Vector:
Expression Vector pCMV3-C-His
Other formats:
Estimated Shipping: 20 Business Days
Toll Free North America
866-819-4732
866-819-4732For Research Use Only
Overview
Description:
Human H6PD / G6PDH Expression-Ready ORF Clone in Mammalian Expression Vector pCMV3-C-His, with C-Terminal His tag. Approximately 10 ug per unit.
Vector:
Expression Vector pCMV3-C-His
Other formats:
Specifications
Type
Expression-Ready ORF Clone
Synonyms
H6PD | 6-phosphogluconolactonase | CORTRD1 | Glucose dehyrogenase | G6PD, H form | Glucose dehydrogenase | G6PDH | Glucose 1- dehydrogenase | GDH
Species
Human
Insert Size
2376 bp
Cloning Enzymes Used
This construct is not yet assembled.
Presentation
Lyophilized
Reconstitution
- Centrifuge at 5,000 x g for 5 minutes.
- Carefully open the tube and add 100 µl of sterile water to dissolve the DNA.
- Close the tube and incubate for 10 minutes at room temperature.
- Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5,000 x g.
- Store the plasmid at -20°C.
Storage
Store lyophilized at room temperature for up to three months.
Validation
The insert is confirmed by full-length sequencing with the sequencing primers listed.
Restrictions
For research use only. Intended for use by laboratory professionals.
Vector
pCMV3-C-His
Vector Type
Expression Vector
Vector Information
All of the pCMV vectors are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
Enhancer: bp 838-1445
SV40 early promoter: bp 2405-2774
Hygromycin ORF: bp 2792-3817
pUC origin: bp 4460-5133
Kanamycin ORF: bp 5207-6022
- Human enhanced cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells.
- Hygromycin resistance gene for selection in mammalian cell lines.
- A Kozak consensus sequence to enhance mammalian expression.
Enhancer: bp 838-1445
SV40 early promoter: bp 2405-2774
Hygromycin ORF: bp 2792-3817
pUC origin: bp 4460-5133
Kanamycin ORF: bp 5207-6022
Expression System
Mammalian
Expression Method
Constitutive, Stable/Transient
Promoter
CMV
Sequencing Primers
T7 - 5' TAATACGACTCACTATAGGG 3'
BGH - 5' TAGAAGGCACAGTCGAGG 3'
Vector Size
6164 bp
Vector Sequence
1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC
51 TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT
101 GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG
151 GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG
201 CTGCTTCGCG AGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG
251 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT
301 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT
351 GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT
401 GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT
451 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT
501 CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC
551 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT
601 ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG
651 TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT
701 CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG
751 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT
801 AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG
851 TCAGATCCTC ACTCTCTTCC GCATCGCTGT CTGCGAGGGC CAGCTGTTGG
901 GCTCGCGGTT GAGGACAAAC TCTTCGCGGT CTTTCCAGTA CTCTTGGATC
951 GGAAACCCGT CGGCCTCCGA ACGGTACTCC GCCACCGAGG GACCTGAGCG
1001 AGTCCGCATC GACCGGATCG GAAAACCTCT CGAGAAAGGC GTCTAACCAG
1051 TCACAGTCGC AAGGTAGGCT GAGCACCGTG GCGGGCGGCA GCGGGTGGCG
1101 GTCGGGGTTG TTTCTGGCGG AGGTGCTGCT GATGATGTAA TTAAAGTAGG
1151 CGGTCTTGAG ACGGCGGATG GTCGAGGTGA GGTGTGGGTT TAGTGAACCG
1201 TCAGATCCTC ACTCTCTTCC GCATCGCTGT CTGCGAGGGC CAGCTGTCAG
1251 GCTTGAGATC CAGCTGTTGG GGTGAGTACT CCCTCTCAAA AGCGGGCATT
1301 ACTTCTGCGC TAAGATTGTC AGTTTCCAAA AACGAGGAGG ATTTGATATT
1351 CACCTGGCCC GATCTGGCCA TACACTTGAG TGACAATGAC ATCCACTTTG
1401 CCTTTCTCTC CACAGGTGTC CACTCCCAGG TCCAAGTTTA AACTTTAATA
1451 CGACTCACTA TAGGGGCCGC CACCAAGCTT GGTACCGCTA GCGGATCCGT
1501 TAACCTTAAG ACCGGTATGG GCTGGTCCTG CATCATCCTG TTCCTCGTGG
1551 CGACCGCGAC CGGGGTCCAC AGCGATATCA TCGATAGCGC TCCCGGGGGT
1601 GGAGGCTCTC ACCATCACCA CCATCATCAC CACCATCACT AAACTCGAGT
1651 CTAGAGCGGC CGCCGAATTC GGGCCCGTTT AAACCCGCTG ATCAGCCTCG
1701 ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC
1751 TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG
1801 AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT
1851 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA
1901 TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT
1951 GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG
2001 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT
2051 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCAG
2101 GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT
2151 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC
2201 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG
2251 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC
2301 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC
2351 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT
2401 AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC
2451 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG
2501 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA
2551 TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC
2601 CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT
2651 TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA
2701 GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCT
2751 CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GATGAAAAAG
2801 CCTGAACTCA CCGCGACGTC TGTCGAGAAG TTTCTGATCG AAAAGTTCGA
2851 CAGCGTCTCC GACCTGATGC AGCTCTCGGA GGGCGAAGAA TCTCGTGCTT
2901 TCAGCTTCGA TGTAGGAGGG CGTGGATATG TCCTGCGGGT AAATAGCTGC
2951 GCCGATGGTT TCTACAAAGA TCGTTATGTT TATCGGCACT TTGCATCGGC
3001 CGCGCTCCCG ATTCCGGAAG TGCTTGACAT TGGGGAATTC AGCGAGAGCC
3051 TGACCTATTG CATCTCCCGC CGTGCACAGG GTGTCACGTT GCAAGACCTG
3101 CCTGAAACCG AACTGCCCGC TGTTCTGCAG CCGGTCGCGG AGGCCATGGA
3151 TGCGATCGCT GCGGCCGATC TTAGCCAGAC GAGCGGGTTC GGCCCATTCG
3201 GACCGCAAGG AATCGGTCAA TACACTACAT GGCGTGATTT CATATGCGCG
3251 ATTGCTGATC CCCATGTGTA TCACTGGCAA ACTGTGATGG ACGACACCGT
3301 CAGTGCGTCC GTCGCGCAGG CTCTCGATGA GCTGATGCTT TGGGCCGAGG
3351 ACTGCCCCGA AGTCCGGCAC CTCGTGCACG CGGATTTCGG CTCCAACAAT
3401 GTCCTGACGG ACAATGGCCG CATAACAGCG GTCATTGACT GGAGCGAGGC
3451 GATGTTCGGG GATTCCCAAT ACGAGGTCGC CAACATCTTC TTCTGGAGGC
3501 CGTGGTTGGC TTGTATGGAG CAGCAGACGC GCTACTTCGA GCGGAGGCAT
3551 CCGGAGCTTG CAGGATCGCC GCGGCTCCGG GCGTATATGC TCCGCATTGG
3601 TCTTGACCAA CTCTATCAGA GCTTGGTTGA CGGCAATTTC GATGATGCAG
3651 CTTGGGCGCA GGGTCGATGC GACGCAATCG TCCGATCCGG AGCCGGGACT
3701 GTCGGGCGTA CACAAATCGC CCGCAGAAGC GCGGCCGTCT GGACCGATGG
3751 CTGTGTAGAA GTACTCGCCG ATAGTGGAAA CCGACGCCCC AGCACTCGTC
3801 CGAGGGCAAA GGAATAGCAC GTGCTACGAG ATTTCGATTC CACCGCCGCC
3851 TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CTGGCTGGAT
3901 GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT
3951 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT
4001 TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA
4051 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA
4101 GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG
4151 CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG
4201 GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC
4251 CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC
4301 CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC
4351 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG
4401 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA
4451 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA
4501 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC
4551 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA
4601 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
4651 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG
4701 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC
4751 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
4801 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG
4851 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG
4901 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
4951 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT
5001 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT
5051 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
5101 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAG CGCGGAACCC
5151 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAAT
5201 TAATTCTTAG AAAAACTCAT CGAGCATCAA ATGAAACTGC AATTTATTCA
5251 TATCAGGATT ATCAATACCA TATTTTTGAA AAAGCCGTTT CTGTAATGAA
5301 GGAGAAAACT CACCGAGGCA GTTCCATAGG ATGGCAAGAT CCTGGTATCG
5351 GTCTGCGATT CCGACTCGTC CAACATCAAT ACAACCTATT AATTTCCCCT
5401 CGTCAAAAAT AAGGTTATCA AGTGAGAAAT CACCATGAGT GACGACTGAA
5451 TCCGGTGAGA ATGGCAAAAG TTTATGCATT TCTTTCCAGA CTTGTTCAAC
5501 AGGCCAGCCA TTACGCTCGT CATCAAAATC ACTCGCATCA ACCAAACCGT
5551 TATTCATTCG TGATTGCGCC TGAGCGAGAC GAAATACGCG ATCGCTGTTA
5601 AAAGGACAAT TACAAACAGG AATCGAATGC AACCGGCGCA GGAACACTGC
5651 CAGCGCATCA ACAATATTTT CACCTGAATC AGGATATTCT TCTAATACCT
5701 GGAATGCTGT TTTCCCAGGG ATCGCAGTGG TGAGTAACCA TGCATCATCA
5751 GGAGTACGGA TAAAATGCTT GATGGTCGGA AGAGGCATAA ATTCCGTCAG
5801 CCAGTTTAGT CTGACCATCT CATCTGTAAC ATCATTGGCA ACGCTACCTT
5851 TGCCATGTTT CAGAAACAAC TCTGGCGCAT CGGGCTTCCC ATACAATCGA
5901 TAGATTGTCG CACCTGATTG CCCGACATTA TCGCGAGCCC ATTTATACCC
5951 ATATAAATCA GCATCCATGT TGGAATTTAA TCGCGGCCTA GAGCAAGACG
6001 TTTCCCGTTG AATATGGCTC ATAACACCCC TTGTATTACT GTTTATGTAA
6051 GCAGACAGTT TTATTGTTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT
6101 TCCACTGAGC GTCAGACCCC GTAGAAATCC GCGCACATTT CCCCGAAAAG
6151 TGCCACCTGA CGTC
Vector Tag
His, C-terminus
Tag Information
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Alternate Vectors
Cloning Vector pGEM-T | Expression Vector pCMV3-Untagged | Expression Vector pCMV3-C-DDK (Flag) | Expression Vector pCMV3-C-GFP | Expression Vector pCMV3-C-HA | Expression Vector pCMV3-C-Myc | Expression Vector pCMV3-C-OFP | Expression Vector pCMV3-SP-N-DDK (Flag) | Expression Vector pCMV3-SP-N-HA | Expression Vector pCMV3-SP-N-His | Expression Vector pCMV3-SP-N-Myc | Expression Vector pLV-C-GFP
Usage Summary
Most commercially available competent cells are appropriate for propagation of the plasmid, e.g. TOP10, DH5a, and TOP10F’.
About H6PD / G6PDH
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Requested From: United States
Date Requested: 8/6/2020
Date Requested: 8/6/2020
