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Rat PSEN1 / Presenilin 1 ELISA Kit (Sandwich ELISA) - LS-F36665
Catalog
Size
Price
LS-F36665-1
1 plate
$790

Popular PSEN1 / Presenilin 1 Elisa Kits


Product Description

LS-F36665 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat PSEN1 / Presenilin 1 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of PSEN1 / Presenilin 1 as low as 75 picograms per milliliter.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
PSEN1 / Presenilin 1
Synonyms
PSEN1, AD3, Alzheimer disease 3, FAD, Protein S182, PS1, S182, PS-1, PSNL1, Presenilin 1, Presenilin-1
Reactivity
Rat
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
125 - 8000 pg/ml
Sensitivity
75 pg/ml
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Store at 4°C. Stable for 6 months.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PS1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PS1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PS1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PS1 can be calculated.
Restrictions
For research use only.
About PSEN1 / Presenilin 1
Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. P49768 NM_000021 NP_000012.1


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Sandwich ELISA Platform Overview
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Sandwich ELISA Platform Overview
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Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 10/23/2018

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