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Rat NT5C3B / NT5C3L ELISA Kit (Competitive EIA) - LS-F39470

Catalog Size Price
LS-F39470-1 1 plate Unavailable

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Rat NT5C3B / NT5C3L ELISA Kit (Competitive EIA) - LS-F39470
Quantitative Competitive EIA
96-Well Strip Plate
Rat
0.312-20 ng/ml
Colorimetric - 450nm (TMB)
Serum, Tissue Homogenates, Plasma

100% Guaranteed
LS-F39470
Competitive EIA (enzyme immunoassay) kit
NT5C3B / NT5C3L
Rat
LS-F39470 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat NT5C3B / NT5C3L in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of NT5C3B / NT5C3L as low as 0.188 nanograms per millilter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
0.312 - 20 ng/ml
0.188
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP Streptavidin Conjugate Diluent
  • Sample Diluent
  • Standard (Lyophilized)
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Adhesive Plate Sealers
    This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Nt5c3l. During the reaction, Nt5c3l in the sample or standard competes with a fixed amount of Nt5c3l on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Nt5c3l. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Nt5c3l in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Short term: 4°C; Long term: see manual.
For research use only.

About NT5C3B / NT5C3L

Q969T7 NP_443167.4

NT5C3B ElisaKit, CN-III-like protein ElisaKit, NT5C3L ElisaKit

Specifically hydrolyzes 7-methylguanosine monophosphate (m7GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m7GMP may protect cells against undesired salvage of m7GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates.

 

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Requested From: 
Date Requested: 5/23/2018

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