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Rat IL32 ELISA Kit (Sandwich ELISA) - LS-F36756

Catalog Size Price
LS-F36756-1 1 plate Unavailable

Most Popular IL32 Elisa Kits

Human IL32 ELISA Kit (Sandwich ELISA) - LS-F5737
Sandwich ELISA
96-Well Strip Plate
Human
15.63-1000 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma
Human IL32 ELISA Kit (Sandwich ELISA) - LS-F11787
Quantitative Sandwich ELISA
96-Well Strip Plate
Human
15.6-1000 pg/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
Human IL32 ELISA Kit (Sandwich ELISA) - LS-F22937
Quantitative Sandwich ELISA
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15.625-1000 pg/ml
Colorimetric - 450nm (TMB)
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Rat IL32 ELISA Kit (Sandwich ELISA) - LS-F23206
Quantitative Sandwich ELISA
96-Well Microplate
Rat
15.625-1000 pg/ml
Colorimetric - 450nm (TMB)
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Human IL32 ELISA Kit (CLIA) - LS-F26283
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100% Guaranteed
LS-F36756
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
IL32
Rat
LS-F36756 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat IL32 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of IL32 as low as 9.375 picograms per milliliter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
15.625 - 1000 pg/ml
9.375
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-32 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-32 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-32 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-32 can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.

About IL32

P24001 NM_004221 NP_004212.4

IL32 ElisaKit, IL-32alpha ElisaKit, IL-32delta ElisaKit, IL-32gamma ElisaKit, Interleukin 32 ElisaKit, Interleukin-32 small ElisaKit, Interleukin-32 theta ElisaKit, Interleukin-32 ElisaKit, Interleukin-32 eta ElisaKit, Natural killer cells protein 4 ElisaKit, IL-32beta ElisaKit, TAIF ElisaKit, TAIFb ElisaKit, TAIFc ElisaKit, TAIFd ElisaKit, NK4 ElisaKit, IL-32 ElisaKit, TAIFa ElisaKit

IL32 is a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

 

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 5/24/2018

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