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Rat FASLG / Fas Ligand ELISA Kit (Sandwich ELISA) - LS-F24923

Ordering

Size Price
1 plate $550
Inquire for larger quantities
LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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LS-F24923
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
FASLG / Fas Ligand
Rat
For the detection and quantification of endogenous levels of natural and/or recombinant Rat CD178 proteins.
LS-F24923 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat FASLG / Fas Ligand in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of FASLG / Fas Ligand as low as 10 picograms per milliliter.
All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.
Cell Culture Supernatants, Plasma, Serum
96-Well Strip Plate
Colorimetric - 450nm
Quantitative
31.2 - 2000 pg/ml
10 pg/ml
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping. ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Coated 96-well Strip Plate
  • Standard (Lyophilized)
  • Biotinylated Detection Antibody (100x)
  • Avidin-Biotin-HRP Complex (100x)
  • Sample Diluent
  • Biotinylated Detection Antibody Diluent
  • Avidin-Biotin-HRP Complex Diluent
  • Wash Buffer (20x)
  • TMB Substrate
  • Stop Solution
  • Adhesive Plate Sealers
    FAS Ligand (FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M (r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9% at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.
Store at 4°C for 6 months or at -20°C for one year. Avoid freeze/thaw cycles.
For research use only.

About FASLG / Fas Ligand

P48023 NM_000639 NP_000630.1

FASLG Elisa Kit, ALPS1B Elisa Kit, Apoptosis antigen ligand Elisa Kit, APTL Elisa Kit, CD95L Elisa Kit, CD95 ligand Elisa Kit, CD178 antigen Elisa Kit, CD95-L Elisa Kit, Fas antigen ligand Elisa Kit, Fas ligand Elisa Kit, FASL Elisa Kit, TNFSF6 Elisa Kit, APT1LG1 Elisa Kit, CD178 Elisa Kit

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.

Requested From: United States
Date Requested: 12/5/2016

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