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Rat F9 / Factor IX ELISA Kit (Sandwich ELISA) - LS-F36736

Catalog Size Price
LS-F36736-1 1 plate Unavailable

Most Popular F9 / Factor IX Elisa Kits

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100% Guaranteed
LS-F36736
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
F9 / Factor IX
Rat
LS-F36736 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat F9 / Factor IX in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of F9 / Factor IX as low as 0.094 nanograms per millilter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
0.156 - 10 ng/ml
0.094
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
    This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- FIX antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-FIX antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FIX amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FIX can be calculated.
Short term: 4°C; Long term: see manual.
For research use only.

About F9 / Factor IX

P00740 NM_000133 NP_000124.1

F9 ElisaKit, Christmas Disease ElisaKit, Coagulation factor IX ElisaKit, Factor IX Deficiency ElisaKit, FIX ElisaKit, HEMB ElisaKit, F9 p22 ElisaKit, Factor 9 ElisaKit, Factor IX ElisaKit, Factor IX F9 ElisaKit, FIX F9 ElisaKit, Haemophilia B ElisaKit, THPH8 ElisaKit, Christmas factor ElisaKit, Hemophilia B ElisaKit, PTC ElisaKit, Serine protease ElisaKit

F9 / Factor IX is a vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII.

 

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 5/22/2018

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