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Rat CYP1A2 ELISA Kit (Sandwich ELISA) - LS-F39026
Catalog
Size
Price
LS-F39026-1
1 plate
$720
Available Discounts
10% off 5+ units

Product Description

LS-F39026 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat CYP1A2 in samples of Cell Lysates and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CYP1A2 as low as 0.118 nanograms per millilter.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
CYP1A2
Synonyms
CYP1A2, Aryl hydrocarbon hydroxylase, Cytochrome P(3)450, Cytochrome P450 1A2, Cytochrome P450 4, p450 form 4, p3-450, p450(PA), CP12, CYPIA2, Cytochrome p450 ia2, Cytochrome P450-P3, Dioxin-inducible P3-450
Reactivity
Rat
Manual
Intended Sample Types
Cell Lysates, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
0.312 - 20 ng/ml
Sensitivity
0.118
Assay Time
4.5 h
Precision
Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Coated 96-well Strip Plate
  • Adhesive Plate Sealers
  • Sample Diluent
  • Assay Diluent A
  • Assay Diluent B
  • Wash Buffer (30x)
  • TMB Substrate
  • Stop Solution
  • Standard (Lyophilized)
  • Detection Reagent A (Green)
  • Detection Reagent B (Red)
Restrictions
For research use only.
About CYP1A2
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Most active in catalyzing 2-hydroxylation. Caffeine is metabolized primarily by cytochrome CYP1A2 in the liver through an initial N3-demethylation. Also acts in the metabolism of aflatoxin B1 and acetaminophen. P05177 NM_000761 NP_000752.2


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Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 10/19/2018

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