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Rat BrdU ELISA Kit (Competitive EIA) - LS-F39437

Catalog Size Price
LS-F39437-1 1 plate Unavailable

Most Popular BrdU Elisa Kits

Rat BrdU ELISA Kit (Competitive EIA) - LS-F27891
Quantitative Competitive EIA
96-Well Strip Plate
Rat
5-100 pmol/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
Rat BrdU ELISA Kit (Competitive EIA) - LS-F39437
Quantitative Competitive EIA
96-Well Strip Plate
Rat
1.56-100 pmol/ml
Colorimetric - 450nm (TMB)
Serum, Tissue Homogenates, Plasma

100% Guaranteed
LS-F39437
Competitive EIA (enzyme immunoassay) kit
BrdU
Rat
LS-F39437 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rat BrdU in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of BrdU as low as 0.938 picomoles per milliliter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
1.56 - 100 pmol/ml
0.938
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP Streptavidin Conjugate Diluent
  • Sample Diluent
  • Standard (Lyophilized)
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Adhesive Plate Sealers
    This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with BrdU. During the reaction,BrdU in the sample or standard competes with a fixed amount ofBrdU on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to BrdU. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of BrdU in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Short term: 4°C; Long term: see manual.
For research use only.

About BrdU

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. 5-Bromodeoxycytidine is deaminated to form BrdU. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle during which DNA is replicated), substituting for thymidine during DNA replication.

 

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Requested From: 
Date Requested: 5/25/2018

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