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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Pig IL4 ELISA Kit (Sandwich ELISA) - LS-F36684
LS-F36684 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Pig IL4 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of IL4 as low as 46.875 picograms per milliliter.
Catalog
Size
Price
LS-F36684-1
1 plate
$690

Popular IL4 Elisa Kits

Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Chicken
Range: 15.6-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Pig
Range: 1.6-400 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Plasma
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rhesus monkey
Range: 7.8-500 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rabbit
Range: 15.63-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Cell Lysates, Serum, Tissue Homogenates, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Mouse
Range: 0.819-200 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Culture Supernatants, Serum, Plasma

Product Description

LS-F36684 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Pig IL4 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of IL4 as low as 46.875 picograms per milliliter.
About IL4
IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. P05112 NM_000589 NP_000580.1

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Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
IL4
Reactivity
Pig
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
78.125 - 5000 pg/ml
Sensitivity
46.875
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofIL-4 can be calculated.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 8/21/2018

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