Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
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LS-F26274 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse IL25 / IL17E in samples of Plasma and Serum. It is based upon a Sandwich CLIA assay principle and can be used to detect levels of IL25 / IL17E as low as 0.56 picograms per milliliter.
All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.
Plasma, Serum
96-Well Strip Plate
Chemiluminescent
Quantitative
1.37 - 1000 pg/ml
0.56 pg/ml
4.5 h
Intra-Assay: CV<10% Inter-Assay: CV<12%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Adhesive Plate Sealers
Coated 96-well Strip Plate
Standard (Lyophilized)
Sample Diluent
Assay Diluent A
Assay Diluent B
Detection Reagent A (Green)
Detection Reagent B (Red)
Wash Buffer (30x)
Substrate A
Substrate B
The Standard, Detection Reagent A, Detection Reagent B, and the 96-Well Strip Plate should be stored at -20°C upon receipt while other components should be at 4°C.
The IL17 proteins are a family of potent cytokines that act to induce proinflammatory responses. Studies have shown that IL17E binds strongly to IL17RB. Receptor binding of ligand has been shown to lead to the activation of nuclear factor kappa-B and production of IL8.
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