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Mouse IL12 ELISA Kit (Sandwich ELISA) - LS-F9981

Catalog Size Price
LS-F9981-1 1 plate Unavailable
Available Discounts 10% off 5+ units

Most Popular IL12 Elisa Kits

Human IL12 ELISA Kit (Sandwich ELISA) - LS-F569
Sandwich ELISA
12 x 8-Well Microstrips
Human
32-2000 pg/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Serum, Plasma
 Image
Mouse IL12 ELISA Kit (Sandwich ELISA) - LS-F610
Sandwich ELISA
12 x 8-Well Microstrips
Mouse
16-1000 pg/ml
Colorimetric - 450nm (TMB)
Cell Lysates, Serum, Plasma
 Image
Human IL12 ELISA Kit (Sandwich ELISA) - LS-F2484
Quantitative Sandwich ELISA
96-Well Microplate
Human
7.8-500 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Serum
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Rat IL12 ELISA Kit (Sandwich ELISA) - LS-F23156
Quantitative Sandwich ELISA
96-Well Microplate
Rat
15.63-1000 pg/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
 Image
Chicken IL12 ELISA Kit (Sandwich ELISA) - LS-F24289
Quantitative Sandwich ELISA
96-Well Microplate
Chicken
12.5-800 pg/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
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100% Guaranteed
LS-F9981
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
IL12
Mouse
This assay has high sensitivity and excellent specificity for detection of mouse IL-12. No significant cross-reactivity or interference between mouse IL-12 and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between mouse IL-12 and all the analogs, therefore, cross reaction may still exist.
LS-F9981 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse IL12 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of IL12 as low as 6.97 picograms per milliliter.
Plasma, Serum, Tissue Homogenates
50 - 100 µl
96-Well Microplate
Quantitative
15.625 - 1000 pg/ml
6.97 pg/ml
Intra-assay: CV%<8% Inter-assay: CV%<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Assay plate (12 x 8 coated Microwells)
  • Standard (Lyophilized)
  • Biotin-antibody (100 x concentrate)
  • HRP-avidin (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin Diluent
  • Sample Diluent
  • Wash Buffer (25 x concentrate)
  • TMB Substrate
  • Stop Solution
  • Adhesive Strip (For 96 wells)
    This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
2°C to 8°C
For research use only.

About IL12

IL-12 ElisaKit


 

Sandwich ELISA Platform Overview

Requested From: 
Date Requested: 12/13/2017

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