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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Mouse/Human/Rat BRAF / B-Raf ELISA Kit (Cell-Based ELISA) - LS-F1832
Catalog
Size
Price
LS-F1832-1
1 plate
$490

Popular BRAF / B-Raf Elisa Kits

Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 0.312-20 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Cell Lysates, Tissue Homogenates
Type: Quantitative Sandwich ELISA
Format: 96-Well Microplate
Reactivity: Human
Range: 0.313-20 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Qualitative Cell-Based Phosphorylation ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Range: Positive/Negative
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Qualitative Cell-Based Phosphorylation ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human
Range: Positive/Negative
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures
Type: Qualitative Cell-Based Phosphorylation ELISA
Format: 96-Well Microplate
Reactivity: Mouse, Human, Rat
Range: Positive/Negative
Detection: Colorimetric - 450nm (TMB)
Sample Types: Adherent Cell Cultures

Product Description

LS-F1832 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Qualitative detection of Human BRAF / B-Raf in samples of Adherent Cell Cultures. It is based upon a Cell-Based assay principle.
About BRAF / B-Raf
BRAF / B-Raf is a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. P15056 NM_004333 NP_004324.2

BRAF ElisaKit, 94 kDa B-raf protein ElisaKit, B-raf ElisaKit, BRAF1 ElisaKit, B Raf ElisaKit, Proto-oncogene B-Raf ElisaKit, p94 ElisaKit, B-RAF1 ElisaKit, NS7 ElisaKit, ONCOGENE BRAF ElisaKit, RAFB1 ElisaKit


Specifications

Type
Cell-Based ELISA (enzyme-linked immunosorbent assay) kit
Target
BRAF / B-Raf
Reactivity
Mouse, Human, Rat
Specificity
B-Raf
Manual
Intended Sample Types
Adherent Cell Cultures
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Qualitative
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • 96-Well Cell Culture Clear-Bottom Microplate
  • 10x TBS
  • Quenching Buffer
  • Blocking Buffer
  • 10x Wash Buffer
  • 100x Anti-B-RAF Antibody (Rabbit Polyclonal)
  • 100x Anti-GAPDH Antibody (Mouse Monoclonal)
  • HRP-Conjugated Anti-Rabbit IgG Antibody
  • HRP-Conjugated Anti-Mouse IgG Antibody
  • Primary Antibody Diluent
  • Ready-to-Use Substrate
  • Stop Solution
  • Crystal Violet Solution
  • SDS Solution
  • Adhesive Plate Seals
Protocol
                Restrictions
                For research use only.

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                Images

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Cell-Based ELISA Platform Overview
                Cell-Based ELISA Platform Overview

                Requested From: United States
                Date Requested: 9/21/2018

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