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Mouse DHEAS / DHEA Sulfate ELISA Kit (Competitive EIA) - LS-F39263

Catalog Size Price
LS-F39263-1 1 plate Unavailable

Most Popular DHEAS / DHEA Sulfate Elisa Kits

Mouse DHEAS / DHEA Sulfate ELISA Kit (Competitive EIA) - LS-F25773
Quantitative Competitive EIA
96-Well Strip Plate
Mouse
6.25-400 ng/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
Rat DHEAS / DHEA Sulfate ELISA Kit (Competitive EIA) - LS-F25817
Quantitative Competitive EIA
96-Well Strip Plate
Rat
31.25-2000 pg/ml
Colorimetric - 450nm (TMB)
Serum, Plasma
Human DHEAS / DHEA Sulfate ELISA Kit (Competitive EIA) - LS-F39260
Quantitative Competitive EIA
96-Well Strip Plate
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15.625-1000 ng/ml
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Mouse DHEAS / DHEA Sulfate ELISA Kit (Competitive EIA) - LS-F39263
Quantitative Competitive EIA
96-Well Strip Plate
Mouse
6.25-400 ng/ml
Colorimetric - 450nm (TMB)
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Rat DHEAS / DHEA Sulfate ELISA Kit (Competitive EIA) - LS-F39264
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100% Guaranteed
LS-F39263
Competitive EIA (enzyme immunoassay) kit
DHEAS / DHEA Sulfate
Mouse
LS-F39263 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse DHEAS / DHEA Sulfate in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of DHEAS / DHEA Sulfate as low as 3.75 nanograms per millilter.
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
Quantitative
6.25 - 400 ng/ml
3.75
Intra-Assay: CV<8% Inter-Assay: CV<10%
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP Streptavidin Conjugate Diluent
  • Sample Diluent
  • Standard (Lyophilized)
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Adhesive Plate Sealers
    This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with DHEA-S. During the reaction, DHEA-S in the sample or standard competes with a fixed amount of DHEA-S on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to DHEA-S. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DHEA-S in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Short term: 4°C; Long term: see manual.
For research use only.

About DHEAS / DHEA Sulfate

DHEAS ElisaKit

Dehydroepiandrosterone sulfate is produced by the addition of a sulfate group, catalyzed by the sulfotransferase enzymes SULT1A1 and SULT1E1, which also produce estrone sulfate from estrone. DHEA sulfate can also be back-converted to DHEA through the action of steroid sulfatase. In the zona reticularis layer of the adrenal cortex, DHEA-sulfate is generated by SULT2A1. This layer of the adrenal cortex is thought to be the primary source of serum DHEA-sulfate.

 

Competition ELISA Platform Overview
Competition ELISA Platform Overview

Requested From: 
Date Requested: 5/24/2018

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